T4 DNA Ligase, expressed in E. coli

Quantity:

Gene target

DNA Ligase expressed
Gene symbol

PRKDC, HLA-DOA
Short name

T4 DNA Ligase, expressed in
Technique

dna
Host

e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Glentham LS supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
Species

E. coli, E. coli
Alternative name

T4 Desoxyribonucleic acid Ligase, expressed in E. coli
Alternative technique

escherichia

Quantity:

Name

Ligase Chain Reaction
Concept

Scope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
Tree numbers
- E05.393.620.311
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data