E. coli DNA Ligase
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Catalog numberM1217-100
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PricePlease ask
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Size1000 U (100 µl)
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DescriptionA NAD+ dependent enzyme that catalyzes the formation of a phosphodiester bond between cohesive 3′-hydroxyl and 5′-phosphoryl termini of double-stranded DNA (dsDNA).. E. coli DNA Ligase is an NAD+ dependent enzyme that catalyzes the formation of a phosphodiester bond between cohesive 3′-hydroxyl and 5′-phosphoryl termini of double-stranded DNA (dsDNA)
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Product Highlights1. Ligation of dsDNA with cohesive termini 2. cDNA cloning of products from second strand cDNA synthesis experiments 3. Alternative to T4 DNA Ligase when blunt-end ligation is not required 4. For ligation of blunt-end fragments use T4 DNA Ligase
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Storage Temp-20°C
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Shippinggel pack
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Shelf Life12 months
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NotesFor research use only. Not to be used for human or animal treatment or consumption.
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Gene target
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Gene symbolPRKDC, HLA-DOA
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Short nameDNA Ligase
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Techniquedna
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Hoste. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. Biovision supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
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SpeciesE. coli, E. coli
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Alternative nameE. coli Desoxyribonucleic acid Ligase
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Alternative techniqueescherichia
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Gene info
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Identity
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Gene
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Long gene nameprotein kinase, DNA-activated, catalytic subunit
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Synonyms gene
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Synonyms gene name
- protein kinase, DNA-activated, catalytic polypeptide
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-11-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class II, DO alpha
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.
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Tree numbers
- E05.393.620.311
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data