MegaMix-Royal - Hot-Start TAQ PCR Mix w/ Blue Dye, 5 x 1 ml
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Catalog number2MMR-05
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Price:
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Size5 x 1 ml
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PropertiesBlue has a wavelength of around 480 nm and will make your sample visible. If your sample is too concentrated you can dissolve it in water. Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. Hot start taq polymerases can be supplied as mix with dNTPs or Mg+ free Polymerases. The units are redifined for reactions but you can delute more this Hot start TAQ polymerase. Storage at -25 º C.
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ConjugationBlue Substrate
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GroupPCR, polymerase chain reaction
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AboutTAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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Gene target
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Short nameMegaMix-Royal - Hot-Start TAQ PCR w/ Dye, 5 x 1
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TechniquePCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. gel company TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
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LabelBlue
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Alternative nameMegaMix-Royal - Hot-Start TAQ PCR test kit Mix w/ Blue Dye, 5 x 1 milliliter
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Alternative techniquedna-amplification
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MeSH Data
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Name
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ConceptScope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
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Tree numbers
- E05.393.620
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data