Hot Start Taq 2x Master Mix - 500 Reactions

Description

Hot start Taq DNA Polymerase 2x master mix is ready to use premix which contains hot start Taq DNA Polymerase, dNTPs, MgCl2 and stabilizers with optimized reaction buffer. Intact Genomics Hot Start Taq 2x Master Mix has been optimized for routine PCR applications. Hot start Taq is a thermostable DNA polymerase that possesses a 5´→3´ polymerase activity (1, 2) and a 5´ flap endonuclease activity (3, 4). Hot Start Taq DNA Polymerase is chemically modified that leads to complete inactivation of the polymerase until the initial heat activation step at the start of PCR. Hot start PCR reduces non-specific amplification during setup stages of the reaction and helps increase PCR specificity and sensitivity. This product is supplied with the unique Intact Genomics 5X Magic Enhancer that enables efficient amplification of GC rich templates up to 84% Product Includes: Hot start Taq 2x master mix 5X Magic Enhancer
Shipping Condition

Ships with Dry Ice
Properties

Hot start taq polymerases can be supplied as mix with dNTPs or Mg+ free Polymerases. The units are redifined for reactions but you can delute more this Hot start TAQ polymerase. Storage at -25 º C. Master mixes of Taq polymerases are supplied with dNTP's and Mg+buffer already premixed. They are used for automation and to exclude variaions in your PCR.

Gene target

Hot Start Taq Master 500
Short name

Hot Start Taq 2x Master - 500
Technique

Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. Intact Genomics TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
Alternative name

Hot Start Taq 2x Master Mix - 500 Reactions