Rabbit HIV1 gp120 antibody (FITC)
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Catalog number
60-00402-3
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Price
Please ask
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Size
50 ug
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Applications
ELISA, WB
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Product Type
Primary Antibodies
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Product Subtype
Conjugated Polyclonal Antibodies
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Research Area
Infectious Disease
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Immunogen
HIV1 gp120 antibody (FITC) was raised in rabbit using full length recombinant gp120 (HIV-1) expressed in baculovirus expression system as the immunogen.
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Specificity
HIV-1 gp120
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Cross Reactivity
To be determined by end-user
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Clone
NA
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Concentration
NA
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Form Buffer
Supplied lyophilized from PBS buffer, with no preservatives added, reconstitute in sterile distilled water.
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Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping Info
Blue Ice
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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About
Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
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French translation
anticorps
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Gene target
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Short name
Rabbit HIV1 gp120 antibody (FITC)
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Technique
Antibody, Rabbit, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Host
Rabbit, Rabbits
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Isotype
IgG2b
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Label
FITC
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Alternative name
Rabbit polyclonal HIV1 gp120 antibody (FITC) conjugated
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Alternative technique
antibodies, rabbit-anti, fluorescine
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Virus
hiv1
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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