HIV1 gp120 antibody FITC
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Catalog number60-00402-3
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PricePlease ask
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Size50 µg
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Polyclonal Antibodies
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Area of researchInfectious Disease
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Type of ImmunogenHIV1 gp120 antibody (FITC) was raised in rabbit using full length recombinant gp120 (HIV-1) expressed in baculovirus expression system ..
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Raised inRabbit
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SpecificityHIV-1 gp120
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Cross ReactivityShould be determined by the end user
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IsotypesIgG2b
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Conjugate TagFITC
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Grade PurityGrater than 95% pure
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Form BufferProvided lyophilized from PBS buffer, with no preservatives added, reconstitute in sterile distilled water.
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StorageStore at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forELISA; WB
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Usage RecommendationsELISA: 1:1,000, WB: 1:500-1:1,000
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Short nameHIV1 gp120 antibody FITC
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative nameRabbit polyclonal HIV1 gp120 antibody (FITC) conjugated
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Alternative techniqueantibodies, fluorescine
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Virushiv1
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data