Rabbit anti Bovine IgG HRP
-
Catalog number
43C-CB0120
-
Price
Please ask
-
Size
1.5 mg
-
-
Category
Secondary Antibody
-
Antibody Subtype
Conjugated Secondary Antibodies
-
Area of research
Immunology
-
Type of Immunogen
Rabbit anti-bovine IgG (HRP) was raised in rabbit using bovine IgG F(c) fragment ..
-
Raised in
Rabbit
-
Specificity
Bovine IgG
-
Cross Reactivity
Should be determined by the end user
-
Conjugate Tag
HRP
-
Method of Purification
Rabbit anti Bovine IgG (HRP) was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
-
Form Buffer
Lyophilized from 0.02M K3PO4, pH 7.2, with 0.15M NaCl, 10 mg/ml PEG, and 0.01% gentamicin sulfate. DO NOT add NaN3.
-
Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
-
Shipping conditions
Blue Ice
-
Tested for
ELISA; IHC; WB
-
Usage Recommendations
ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,500, WB: 1:1,000-1:10,000
-
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
-
Conjugation
HRP Conjugated
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
-
Short name
Rabbit anti IgG
-
Technique
Rabbit, anti, IgG, antibody to, IgGs
-
Host
Rabbit, Rabbits
-
Isotype
IgG, IgG
-
Label
HRP
-
Species
Bovine, Bos Taurus genes are available form the Bovine Genome database.
-
Alternative name
Rabbit anti Bovine IgG secondary antibody (HRP)
-
Alternative technique
rabbit-anti, antibodies, immunoglobulins
-
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products