Rabbit anti Bovine IgG
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Catalog number40C-CB0115
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Price:
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Size2 mg
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CategorySecondary Antibody
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Antibody SubtypePurified Secondary Antibodies
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Area of researchImmunology
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Type of ImmunogenRabbit anti-bovine IgG was raised in rabbit using bovine IgG F(c) fragment ..
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Raised inRabbit
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SpecificityBovine IgG
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Cross ReactivityShould be determined by the end user
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Method of PurificationRabbit anti Bovine IgG was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
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Concentration~2.0 mg/ml
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Form Buffer0.01M Na3PO4, pH 7.2, with 0.14M NaCl, and 0.01% NaN3.
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StorageStore at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forELISA; IHC; WB
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Usage RecommendationsELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by fitzgerald. Rabbit antibodies are very stable and can be stored for several days at room temperature. fitzgerald adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin nameOryctolagus cuniculus
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Short nameRabbit anti IgG
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TechniqueRabbit, anti, IgG, antibody to, IgGs
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HostRabbit, Rabbits
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IsotypeIgG, IgG
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SpeciesBovine, Bos Taurus genes are available form the Bovine Genome database.
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Alternative nameRabbit anti Bovine IgG secondary antibody
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Alternative techniquerabbit-anti, antibodies, immunoglobulins
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data