Perforin antibody biotin
-
Catalog number61R-P116ABT
-
PricePlease ask
-
Size100 µg
-
-
CategoryPrimary Antibody
-
Antibody SubtypeConjugated Monoclonal Antibodies
-
Area of researchImmunology
-
Type of ImmunogenPerforin antibody (biotin) was raised in mouse using purified granules from human YT lymphoma cell line ..
-
Raised inMouse
-
SpecificityHuman
-
Cross ReactivityShould be determined by the end user
-
IsotypesIgG2b
-
Clonedelta G9
-
Conjugate TagBiotin
-
Grade PurityGrater than 95% pure
-
Method of PurificationPerforin antibody (biotin) was purified by Protein A chromatography.
-
Concentration1.0 mg/ml
-
Form Buffer50 mM Na3PO4, pH 7.5, with 100 mM KCL, 150 mM NaCl, 5% Glycerol, 0.2% BSA, and 0.04% NaN3.
-
StorageStore at 4 deg C.
-
Shipping conditionsBlue Ice
-
Tested forFC; IHC
-
Usage RecommendationsFC: 2 ug/ml
-
Additional InformationThis antibody recognizes the human perforin molecule of 70 kDa.
-
PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
-
ConjugationBiotinylated
-
French translationanticorps
-
Gene target
-
Gene symbolPRF1
-
Short namePerforin antibody
-
TechniqueAntibody, antibodies against human proteins, antibodies for
-
LabelBiotin
-
Alternative nameMouse monoclonal Perforin antibody (biotin)
-
Alternative techniqueantibodies
-
Gene info
-
Identity
-
Gene
-
Long gene nameperforin 1
-
Synonyms gene name
- perforin 1 (pore forming protein)
-
Synonyms
-
Synonyms name
-
GenBank acession
-
Locus
-
Discovery year1989-02-23
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
Classification
- C2 domain containing
-
VEGA ID
-
Locus Specific Databases
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data