p47 Treponema pallidum His tag

Category

Proteins
Antibody Subtype

Conjugated Proteins
Area of research

Infectious Disease
Reactivity

Bacterial
Residues

Tpp47 recombinant lipoprotein has been prepared by expressing the complete ORF of the corresponding gene which codifies the mature lipoprotein of 47 kDa of the spirochete Treponema pallidum
Conjugate Tag

His tag
Type of protein

Recombinant
Expression System

Escherichia coli
Grade Purity

Grater than 95% pure
Concentration

0.5 - 1 mg/ml
Form Buffer

Provided in 20 mM phosphate buffer, pH 8.0, with 1 M NaCl and 0.1% polyoxyethelene (10) tridecyl ether and 6 mM urea
Storage

Store at -20 deg C to -80 deg C, avoid repeated freeze/thaw cycles
Shipping conditions

Dry Ice
Tested for

ELISA; ICC; Dot Blot; WB
Usage Recommendations

ELISA: 1:2000
Bioactivity

Immunoreactive with sera of syphilis-infected individuals
URL
More info
Conjugation

histidine

Name

Fluorescent Treponemal Antibody-Absorption Test
Concept

Scope note: Serologic assay that detects antibodies to Treponema pallidum, the etiologic agent of syphilis. After diluting the patient's serum to remove non-specific antibodies, the serum is mixed on a glass slide with Nichol's strain of Treponema pallidum. An antigen-antibody reaction occurs if the test is positive and the bound antibodies are detected with fluoresceinated antihuman gamma-globulin antibody.
Tree numbers
- E01.370.225.812.735.850.200
- E05.200.812.735.850.200
- E05.478.594.760.850.200
Qualifiers

ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data