p15 Treponema pallidum His tag
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Catalog number
80-1369
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Price
Please ask
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Size
1 mg
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Category
Proteins
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Antibody Subtype
Conjugated Proteins
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Area of research
Infectious Disease
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Reactivity
Bacterial
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Residues
Tpp15 recombinant lipoprotein has been prepared by expressing the complete ORF of the corresponding gene which codifies the mature lipoprotein of 15 kDa of the spirochete Treponema pallidum without the leader peptide
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Conjugate Tag
His tag
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Type of protein
Recombinant
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Expression System
Escherichia coli
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Grade Purity
Grater than 98% pure
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Concentration
0.5 - 1 mg/ml
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Form Buffer
Provided in 20 mM phosphate buffer, pH 8.0, with 1 M NaCl and 0.1% polyoxyethelene (10) tridecyl ether and 10 mM beta-mercaptoethanol
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Storage
Store at -20 deg C to -80 deg C, avoid repeated freeze/thaw cycles
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Shipping conditions
Blue Ice
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Tested for
ELISA; ICC; Dot Blot; WB
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Usage Recommendations
ELISA: 1:1500
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Bioactivity
Immunoreactive with sera of syphilis-infected individuals
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Conjugation
histidine
MeSH Data
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Name
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Concept
Scope note:
Serologic assay that detects antibodies to Treponema pallidum, the etiologic agent of syphilis. After diluting the patient's serum to remove non-specific antibodies, the serum is mixed on a glass slide with Nichol's strain of Treponema pallidum. An antigen-antibody reaction occurs if the test is positive and the bound antibodies are detected with fluoresceinated antihuman gamma-globulin antibody.
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Tree numbers
- E01.370.225.812.735.850.200
- E05.200.812.735.850.200
- E05.478.594.760.850.200
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Qualifiers
ethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data
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