M13 + fd + F1 Filamentous Phages antibody FITC

• Catalog number
  61R-M101aFT
• Price
  Please ask
• Size
  250 µl

• Category
  Primary Antibody
• Antibody Subtype
  Conjugated Monoclonal Antibodies
• Area of research
  Miscellaneous
• Type of Immunogen
  M13 phage antibody (FITC) was raised in mouse using fd phages from E.Coli F+ strain ..
• Raised in
  Mouse
• Specificity
  M13 phage
• Cross Reactivity
  This antibody recognizes an epitope on pVIII (phage coat protein) covering the N-Terminal region of g8p AEGDDPAKAAFDSLQASAT
• Isotypes
  IgG2b
• Clone
  B62-FE2
• Conjugate Tag
  FITC
• Method of Purification
  Protein A affinity chromatography
• Form Buffer
  Tris buffer, pH 8.0, 0.5% BSA, 0.09% NaN3.
• Storage
  Store at 4 deg C.
• Shipping conditions
  Blue Ice
• Tested for
  Please refer to the datasheet
• URL
• More info
• Properties
  If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Gene
  M13 bacteriophage is a virus that infects the bacterium Escherichia coli. It is composed of a circular single-stranded DNA molecule encased in a thin flexible tube made up of about 2700 copies of a single protein called P8, the major coat protein. The ends of the tube are capped with minor coat proteins. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic or temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.
• French translation
  anticorps

• Gene target
  M13   Filamentous   Phages  
• Short name
  M13 + fd + F1 Filamentous Phages antibody FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Label
  FITC
• Alternative name
  Mouse monoclonal M13 + fd + F1 Filamentous Phages antibody (FITC)
• Alternative technique
  antibodies, fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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