M13 + fd + F1 Filamentous Phages antibody biotin
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Catalog number
61R-M101aBT
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Price
Please ask
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Size
250 µl
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Category
Primary Antibody
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Antibody Subtype
Conjugated Monoclonal Antibodies
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Area of research
Miscellaneous
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Type of Immunogen
M13 phage antibody (biotin) was raised in mouse using fd phages from E. coli F+ strain ..
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Raised in
Mouse
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Specificity
M13 phage
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Cross Reactivity
This antibody recognizes an epitope on pVIII(phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT
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Isotypes
IgG2b
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Clone
B62-FE2
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Conjugate Tag
Biotin
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Method of Purification
Protein A affinity chromatography
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Form Buffer
PBS, pH 7.4, with 0.5% BSA, and 0.09% NaN3
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Storage
Store at 4 deg C.
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Shipping conditions
Blue Ice
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Tested for
ELISA; WB
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Usage Recommendations
ELISA: 1:5,000-1:10,000
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Gene
M13 bacteriophage is a virus that infects the bacterium Escherichia coli. It is composed of a circular single-stranded DNA molecule encased in a thin flexible tube made up of about 2700 copies of a single protein called P8, the major coat protein. The ends of the tube are capped with minor coat proteins. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic or temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.
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French translation
anticorps
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Gene target
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Short name
M13 + fd + F1 Filamentous Phages antibody
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Technique
Antibody, antibodies against human proteins, antibodies for
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Label
Biotin
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Alternative name
Mouse monoclonal M13 + fd + F1 Filamentous Phages antibody (biotin)
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Alternative technique
antibodies
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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