Leptin antibody biotin
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Catalog number61-1010
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PricePlease ask
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Size100 µl
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Monoclonal Antibodies
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Area of researchHormones & Steroids
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Type of ImmunogenLeptin antibodies were raised in Mouse using Recombinant human leptin .
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Raised inMouse
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Specificity61-1010 is specific for human leptin
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Cross ReactivityHuman
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IsotypesIgG1 kappa
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CloneM81396-B
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Conjugate TagBiotin
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Method of PurificationLeptin antibody was purified from cell culture supernatant by protein A/G chromatography
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Form BufferProvided in 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM NaN3
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StorageStore at 4-8 deg C without exposure to light
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Shipping conditionsBlue Ice
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Tested forELISA
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Assay Information61-1010 can be used as the detection antibody in a matched pair immunoassay in conjunction with 10-1757 as the capture antibody
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Additional InformationA non-biotinylated version of this Leptin antibody is also available under catalog no. 10-1759 which can be labelled and used as a detection antibody as per assay requirements
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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GeneHuman or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.
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French translationanticorps
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Gene target
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Gene symbolLEP, LEPR, LEPROT
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Short nameLeptin antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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LabelBiotin
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Alternative nameMouse monoclonal Leptin antibody (Biotin)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameleptin
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Synonyms gene
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Synonyms gene name
- leptin (murine obesity homolog)
- leptin (obesity homolog, mouse)
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Locus
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Discovery year1993-01-26
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Entrez gene record
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Pubmed identfication
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Classification
- Neuropeptides
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameleptin receptor
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-05-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameleptin receptor overlapping transcript
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-11-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
