Leptin antibody biotin
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Catalog number60R-LR026bt
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Price:
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Size50 µg
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Polyclonal Antibodies
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Area of researchHormones & Steroids
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Type of ImmunogenLeptin antibodies were raised in rabbit using highly pure recombinant murine leptin ..
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Raised inRabbit
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SpecificityMurine leptin
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Cross ReactivityShould be determined by the end user
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Conjugate TagBiotin
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Form BufferLyophilized from PBS, pH 7.2. Reconstitute in sterile PBS with 0.1% BSA to a concentration of at least 50ug/ml.
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StorageStore at -20 deg C until reconstitution. Following reconstitution product may be stored at 4 deg C in the short term. For long term storage aliquot and freeze at -20 deg C. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forELISA; WB
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Usage RecommendationsELISA: 0.25-1 ug/ml, WB: 0.1-0.2 ug/ml
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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ConjugationBiotinylated
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GeneHuman or mouse Leptin (from Greek λεπτός leptos, "thin") the "satiety hormone", is a hormone made by adipose cells that helps to regulate energy balance by inhibiting hunger. Leptin is opposed by the actions of the hormone ghrelin, the "hunger hormone". Both hormones act on receptors in the arcuate nucleus of the hypothalamus to regulate appetite to achieve energy homeostasis. ELISA kits and peptides and antibodies are available.
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French translationanticorps
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Gene target
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Gene symbolLEP, LEPR, LEPROT
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Short nameLeptin antibody
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TechniqueAntibody, antibodies against human proteins, antibodies for
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LabelBiotin
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Alternative nameRabbit polyclonal Leptin antibody (biotin)
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameleptin
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Synonyms gene
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Synonyms gene name
- leptin (murine obesity homolog)
- leptin (obesity homolog, mouse)
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Locus
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Discovery year1993-01-26
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Entrez gene record
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Pubmed identfication
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Classification
- Neuropeptides
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameleptin receptor
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Synonyms
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GenBank acession
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Locus
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Discovery year1996-05-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameleptin receptor overlapping transcript
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year2004-11-16
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data