Keratin K5/K8 Pan Epithelial antibody FITC
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Catalog number
61R-1006
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Price
Please ask
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Size
250 µl
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Category
Primary Antibody
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Antibody Subtype
Conjugated Monoclonal Antibodies
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Area of research
Cell Biology
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Type of Immunogen
Keratin K5/K8 (Pan Epithelial) antibody (FITC) was raised in mouse using human keratin K8, purified from SDS PAGE gel ..
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Raised in
Mouse
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Specificity
Human keratin K8
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Cross Reactivity
Bovine, Murine, Amphibia, Pig, Fish
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Isotypes
IgG1
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Clone
C22
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Conjugate Tag
FITC
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Method of Purification
Keratin K5/K8 (Pan Epithelial) antibody (FITC) was purified by Protein A affinity chromatography
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Form Buffer
Provided in liquid form, contains 0.09% NaN3
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Storage
Store at 2-8 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
IHC
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Usage Recommendations
IHC: 1:10
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Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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French translation
anticorps
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Gene target
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Short name
Keratin K5/K8 Pan Epithelial antibody FITC
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Technique
Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Label
FITC
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Alternative name
Mouse monoclonal Keratin K5/K8 (Pan Epithelial) antibody (FITC)
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Alternative technique
antibodies, fluorescine
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MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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