Keratin K5/K8 Pan Epithelial antibody biotin

Category

Primary Antibody
Antibody Subtype

Conjugated Monoclonal Antibodies
Area of research

Cell Biology
Type of Immunogen

Keratin K5/K8 (Pan Epithelial) antibody (biotin) was raised in mouse using human keratin K8, purified from SDS PAGE gel ..
Raised in

Mouse
Specificity

Human keratin K8
Cross Reactivity

Bovine, Murine, Amphibia, Pig
Isotypes

IgG1
Clone

C22
Conjugate Tag

Biotin
Method of Purification

Keratin K5/K8 (Pan Epithelial) antibody (Biotin) was purified by Protein A affinity chromatography
Form Buffer

Provided in liquid form, contains 0.09% NaN3
Storage

Store at 2-8 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
Shipping conditions

Blue Ice
Tested for

ELISA; IHC
Usage Recommendations

IHC: 1:10 - 1:20
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated
French translation

anticorps

Gene target

Keratin K5/K8 Pan Epithelial
Short name

Keratin K5/K8 Pan Epithelial antibody
Technique

Antibody, antibodies against human proteins, antibodies for
Label

Biotin
Alternative name

Mouse monoclonal Keratin K5/K8 (Pan Epithelial) antibody (biotin)
Alternative technique

antibodies

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data