IgM Isotype Control Fc fusion protein (FITC)
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Catalog number
35R-0009
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Price
Please ask
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Size
50 ug
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Research Area
Immunology
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Product Subtype
Isotype Controls
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Product Type
Biological Reagents
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Concentration
200 ug/mL
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Form and Buffer
aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
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Storage
Store at 2-8 deg C. Do not freeze. Protect from light.
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Shipping Info
Blue Ice
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Applications
FC
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Description
Isotype or positive controls by peptides, antibodies and deactivated samples. Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.
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Properties
This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen, Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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Conjugation
Anti-FITC Antibody
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Group
isotypes
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Gene target
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Gene symbol
FCGBP, FCRL2
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Short name
IgM Isotype Control Fc protein (FITC)
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Technique
Control, Isotype, FITC, IgM, FC, controls, Fluorescein, IgMs, Isotypes and controls
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Isotype
IgM, IgM
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Label
FITC
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Alternative name
Immunoglobulin M isoform reference fragment c fusion protein (fluorecein)
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Alternative technique
control, controls, fluorescine, immunoglobulin-m
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Tissue
control
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Gene info
Gene info
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Identity
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Gene
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Long gene name
Fc receptor like 2
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Synonyms gene
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Synonyms gene name
- SH2 domain-containing phosphatase anchor protein 1
- Fc receptor-like 2
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Synonyms
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GenBank acession
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Locus
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Discovery year
2001-04-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Immunoglobulin like domain containing
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VEGA ID
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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