IgG1 Isotype Control Fc fusion protein (biotin)
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Catalog number35R-0022
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Price:
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Size100 ug
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Research AreaImmunology
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Product SubtypeIsotype Controls
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Product TypeBiological Reagents
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Concentration500 ug/ml
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Form and Bufferaqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
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StorageStore at 2-8 deg C. Do not freeze. Protect from light.
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Shipping InfoBlue Ice
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ApplicationsFC
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PropertiesBiotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. Immunoglobulin Isotype specific antibodies and controls are specific to the immunoglobulin heavy chains and immunoglobulin light chains. Mouse monoclonal antibodies from this clone have all the same affinity to the antigen. Also polyclonal antibodies are Isotype purified amd conjugated or if they are used as control non conjugated.
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ConjugationBiotinylated
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DescriptionIsotype or positive controls by peptides, antibodies and deactivated samples. Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.
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Groupisotypes
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Gene target
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Short nameIgG1 Isotype Control Fc protein ( )
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TechniqueControl, Isotype, FC, controls, Isotypes and controls
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IsotypeIgG1
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LabelBiotin
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Alternative nameIgG1 isoform reference fragment c fusion protein (biotinilated)
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Alternative techniquecontrol, controls
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Tissuecontrol
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