Goat anti Rat IgG H + L FITC
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Catalog number
43C-CB1216
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Price
Please ask
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Size
2 mg
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Goat anti-rat IgG (H+L) (FITC) was raised in goat using rat IgG whole molecule ..
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Raised in
Goat
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Specificity
Rat IgG (H + L)
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Cross Reactivity
Should be determined by the end user
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Conjugate Tag
FITC
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Method of Purification
Goat anti Rat IgG (H + L) (FITC) was purified by affinity chromatography.
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Form Buffer
Lyophilized. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. 10 mg/ml Bovine Serum Albumin (BSA)-I mMunoglobulin and Protease free. 0.01% (w/v) NaN3
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Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditions
Blue Ice
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Tested for
FC; IF
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Usage Recommendations
FC: 1:2,000-1:10,000, IF: 1:500-1:2,500
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus, Rattus norvegicus
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Gene target
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Gene symbol
CALD1, FUT1, L3MBTL2
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Short name
Goat anti IgG H + L FITC
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Technique
anti, FITC, Goat, IgG, antibody to, Fluorescein, goats, IgGs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgG, IgG
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Label
FITC
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Species
Rat, Goats, Rats
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Alternative name
Goat anti Rat IgG (H + L) secondary antibody (FITC)
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Alternative technique
antibodies, caprine, fluorescine, immunoglobulins
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Gene info
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Identity
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Gene
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Long gene name
caldesmon 1
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Synonyms
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GenBank acession
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Locus
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Discovery year
1992-07-20
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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VEGA ID
Gene info
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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