Goat anti Rat IgG Fab'2 FITC
-
Catalog number
43C-CJ0318
-
Price
Please ask
-
Size
1 mg
-
-
Category
Secondary Antibody
-
Antibody Subtype
Conjugated Secondary Antibodies
-
Area of research
Immunology
-
Type of Immunogen
Goat anti-rat IgG (Fab'2) (FITC) was raised in goat using rat IgG F(ab')2 fragment ..
-
Raised in
Goat
-
Specificity
Rat IgG (Fab2)
-
Cross Reactivity
Should be determined by the end user
-
Conjugate Tag
FITC
-
Method of Purification
Goat anti Rat IgG (Fab'2) (FITC) was purified by affinity chromatography.
-
Form Buffer
Lyophilized from 0.01M Na3PO4, pH 7.6, with 0.25M NaCl, 15 mg/ml BSA, and 0.01% NaN3. I mMunoglobulin and protease free.
-
Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
-
Shipping conditions
Blue Ice
-
Tested for
ELISA; FC; IF
-
Usage Recommendations
ELISA: 1:10,000-1:50,000, FC: 1:500-1:2,500, IF: 1:1,000-1:5,000
-
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
Properties
Human Fab Of Normal IgG Chromatographically purified Fab fragment of human IgG. Purified human Fab may be used as a reference antigen, calibrator, coating protein and blocking agent, This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
-
Conjugation
Anti-FITC Antibody
-
Latin name
Capra aegagrus hircus, Rattus norvegicus
-
About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
-
Gene target
-
-
Gene symbol
MIR514A2, MIR7-2, MIR329-2
-
Short name
Goat anti IgG Fab'2 FITC
-
Technique
anti, FITC, Goat, Fab, IgG, antibody to, fabs, Fluorescein, goats, IgGs
-
Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
-
Isotype
fab'2, IgG, Fab fragment, IgG
-
Label
FITC
-
Species
Rat, Goats, Rats
-
Alternative name
Goat anti Rat IgG secondary antibody (Fab'2) (FITC)
-
Alternative technique
antibodies, caprine, fluorescine, seconairy, immunoglobulins
-
Gene info
-
Identity
-
Gene
-
Long gene name
microRNA 514a-2
-
Synonyms gene
-
Synonyms gene name
-
Synonyms
-
Locus
-
Discovery year
2005-07-15
-
Entrez gene record
-
RefSeq identity
-
Classification
Gene info
-
Identity
-
Gene
-
Long gene name
microRNA 7-2
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year
2004-04-23
-
Entrez gene record
-
RefSeq identity
-
Classification
Gene info
-
Identity
-
Gene
-
Long gene name
microRNA 329-2
-
Synonyms gene
-
Synonyms
-
Locus
-
Discovery year
2005-07-11
-
Entrez gene record
-
RefSeq identity
-
Classification
MeSH Data
-
Name
-
Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
Similar products