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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Raised in
Goat
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Specificity
Pig
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Cross Reactivity
Should be determined by the end user
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Isotypes
IgG
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Conjugate Tag
FITC
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Method of Purification
Goat anti Pig IgA (FITC) was purified by affinity chromatography.
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Concentration
1.0 mg/ml
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Form Buffer
Provided as a liquid in PBS, pH 7.2, with 0.1% NaN3.
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Storage
Store at 4 deg C.
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Shipping conditions
Blue Ice
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Tested for
FC; ICC; IHC
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Usage Recommendations
ICC: 1:200-1:2,000, IHC: 1:200-1:2,000
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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About
Pigs and the smaller guinea pigs are frequent used as models for humans.
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Short name
Goat anti IgA FITC
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Technique
anti, FITC, Goat, IgA, antibody to, Fluorescein, goats, IgAs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgA, IgA
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Label
FITC
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Species
Pig, Goats, Pigs and Guinea pigs
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Alternative name
Goat anti Pig IgA secondary antibody (FITC)
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Alternative technique
antibodies, caprine, fluorescine, immunoglobulin-a
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