Goat anti Human IgA alpha chain FITC
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Catalog number
43C-CB9108
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Price
Please ask
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Size
1 mg
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Category
Secondary Antibody
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Antibody Subtype
Conjugated Secondary Antibodies
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Area of research
Immunology
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Type of Immunogen
Goat anti-Human IgA (alpha chain) (FITC) was raised in goat using purified Human IgA ..
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Raised in
Goat
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Specificity
This antibody reacts with heavy chains on human IgA (alpha chain) and
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Cross Reactivity
No reactivity is observed to non-immunoglobulin human serum proteins or light chains on all human immunoglobulins.
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Conjugate Tag
FITC
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Method of Purification
Affinity purified using solid phase Human IgA
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Source
Goat serum was obtained from healthy animals of US origin and under the care of a registered veterinarian.
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Concentration
1.0 mg/ml (lot specific)
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Form Buffer
Clear, fluorescent yellow liquid, containing 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
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Storage
Store at 2-8 deg C.
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Shipping conditions
Blue Ice
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Tested for
FC; IF; IHC
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Usage Recommendations
The optimal working dilution should be determined by the end user. Suggested starting dilution(s): 1:20 – 1:2000 for most applications
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Description
The Goat anti IgA alpha chain FITC is a α- or alpha protein sometimes glycoprotein present in blood. This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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Properties
This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples. Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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Gene target
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Gene symbol
CGA, FGA, COL4A4
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Short name
Goat anti IgA alpha chain FITC
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Technique
anti human, anti, FITC, Goat, IgA, antibody to, anti human antibodies, Fluorescein, goats, IgAs
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
IgA, IgA
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Label
FITC
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Species
Human, Goats, Humans
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Alternative name
Goat anti Human IgA (alpha chain) secondary antibody (FITC)
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Alternative technique
antibodies, caprine, fluorescine, immunoglobulin-a
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Gene info
Gene info
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Identity
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Gene
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Long gene name
fibrinogen alpha chain
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Synonyms gene name
- fibrinogen, A alpha polypeptide
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Locus
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Discovery year
2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- Fibrinogen C domain containing
- Receptor ligands
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VEGA ID
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Locus Specific Databases
Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
Product images
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