EBV p18 protein (His tag/Biotin)

Product Type

Proteins
Product Subtype

Conjugated Proteins
Research Area

Infectious Disease
Tag Conjugate

p18 expressed as a recombinant antigen fused to a his-tag in its N-terminus and a biotin molecule in its C-terminus. Produced from the almost complete ORF of the BFRF3 gene (aa 5-176)
Type1

Recombinant
Form Buffer

Supplied in 20 mM phosphate buffer, pH 8.0, with 1 M NaCl and 0.1% polyoxyethelene (10) tridecyl ether and 6 mM urea
Storage

Store at -20 deg C to -80 deg C, avoid repeated freeze/thaw cycles
Applications

ELISA, ICC, Dot Blot, WB
Shipping Info

Blue Ice
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated, histidine