EBV p18 protein His tag/Biotin

• Catalog number
  80-1356
• Price
  Please ask
• Size
  100 µg

• Category
  Proteins
• Antibody Subtype
  Conjugated Proteins
• Area of research
  Infectious Disease
• Reactivity
  Viral
• Residues
  p18 expressed as a recombinant antigen fused to a his-tag in its N-terminus and a biotin molecule in its C-terminus. Produced from the almost complete ORF of the BFRF3 gene (aa 5-176)
• Conjugate Tag
  Biotin
• Type of protein
  Recombinant
• Expression System
  Escherichia coli
• Grade Purity
  Grater than 98% pure
• Concentration
  > 2 mg/ml
• Form Buffer
  Provided in 20 mM phosphate buffer, pH 8.0, with 1 M NaCl and 0.1% polyoxyethelene (10) tridecyl ether and 6 mM urea
• Storage
  Store at -20 deg C to -80 deg C, avoid repeated freeze/thaw cycles
• Shipping conditions
  Blue Ice
• Tested for
  ELISA; ICC; Dot Blot; WB
• Usage Recommendations
  ELISA: 1:3000
• Bioactivity
  Immunoreactive with sera of IgM VCA positive infected individuals
• URL
• More info
• Properties
  Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
• Conjugation
  Biotinylated, histidine

• Gene target
  EBV   p18   protein   His   tag  
• Short name
  EBV p18 protein His tag/
• Technique
  Tags
• Label
  His
• Alternative name
  Purified recombinant EBV p18 protein (His tagged and Biotinylated)
• Virus
  ebv

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