E. coli O157 antibody FITC

• Catalog number
  61C-CR1295M1F
• Price
  Please ask
• Size
  100 µg

• Category
  Primary Antibody
• Antibody Subtype
  Conjugated Monoclonal Antibodies
• Area of research
  Infectious Disease
• Type of Immunogen
  E. coli O157 antibody (FITC) was raised in Mouse using the 'O' antigen of E. coli serotype O157 ..
• Raised in
  Mouse
• Specificity
  E Coli O157
• Cross Reactivity
  This antibody recognizes the 'O' antigen of E coli serotype O157
• Isotypes
  IgG3
• Clone
  1316501
• Conjugate Tag
  FITC
• Grade Purity
  Grater than 90% pure
• Concentration
  Batch dependent
• Form Buffer
  PBS, pH 7.4, with 10 mg/ml BSA and 0.1% NaN3.
• Storage
  Aliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles
• Shipping conditions
  Blue Ice
• Tested for
  ELISA; IF
• Usage Recommendations
  Optimal conditions to be determined by end user
• URL
• More info
• Properties
  If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• French translation
  anticorps

• Gene target
  O157  
• Short name
  O157 antibody FITC
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
• Host
  e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. fitzgerald supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
• Label
  FITC
• Species
  E. coli, E. coli
• Alternative name
  Mouse monoclonal E. coli O157 antibody (FITC)
• Alternative technique
  antibodies, escherichia, fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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