E. coli O157 antibody FITC
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Catalog number61C-CR1295M4F
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Price:
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Size100 µg
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Monoclonal Antibodies
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Area of researchInfectious Disease
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Type of ImmunogenE. coli O157 antibody (FITC) was raised in mouse using 'O' antigen of E. coli serotype O157 ..
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Raised inMouse
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SpecificityE Coli O157
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Cross ReactivityThis antibody recognizes the 'O' antigen of E coli serotype O157 Non-reactive with E coli serotypes: O111, O125, O20, O55 & K14
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IsotypesIgG3
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Clone1616501
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Conjugate TagFITC
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Grade PurityGrater than 90% pure
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ConcentrationBatch dependent
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Form BufferPBS , pH 7.2, with 0.1% NaN3.
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StorageAliquot and store at -20 deg C. Avoid repeated freeze/thaw cycles
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Shipping conditionsBlue Ice
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Tested forELISA; IF
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Usage RecommendationsELISA: 1:20-1:200, IF: 1:10-1:50
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Short nameO157 antibody FITC
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Hoste. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. fitzgerald supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
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LabelFITC
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SpeciesE. coli, E. coli
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Alternative nameMouse monoclonal E. coli O157 antibody (FITC)
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Alternative techniqueantibodies, escherichia, fluorescine
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data