Chicken anti Rat IgG H + L Alk Phos

• Catalog number
  43R-1510
• Price
  Please ask
• Size
  500 µg

• Category
  Secondary Antibody
• Antibody Subtype
  Conjugated Secondary Antibodies
• Area of research
  Immunology
• Type of Immunogen
  Purified Rat IgG (H&L)
• Raised in
  Chicken
• Specificity
  This antibody reacts with heavy (gamma) chains on rat IgG and light chains on all rat immunoglobulins.
• Cross Reactivity
  No reactivity is observed to non-immunoglobulin rat serum proteins, IgG from human or rabbit or serum proteins from human or rabbit.
• Conjugate Tag
  Alkaline Phosphatase
• Grade Purity
  Grater than 95% pure
• Method of Purification
  Affinity purified using solid phase Rat IgG (H&L)
• Source
  Ig fraction was prepared using eggs obtained from healthy hens of US origin.
• Concentration
  1.5 mg/ml (lot specific)
• Form Buffer
  Clear, colorless liquid, containing 30 mM Triethanolamine, 5 mM Magnesium Chloride, 0.1 mM Zinc Chloride, 1% (w/v) BSA and 0.05% (w/v) NaN3. Protease/IgG free, pH 7.2.
• Storage
  Store undiluted liquid at 2-8 deg C. For storage at -20 deg C, aliquot and dilute with an equal volume of glycerol to prevent loss of enzymatic activity.
• Shipping conditions
  Blue Ice
• Tested for
  Please refer to the datasheet
• Usage Recommendations
  1:5000-1:50,000 ELISA/WB, 1:500-1:2000 IHC
• URL
• More info
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
• About
  Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
• Latin name
  Rattus norvegicus

• Gene target
  Alk   Phos  
• Short name
  anti IgG H + L Alk Phos
• Technique
  anti, IgG, antibody to, IgGs
• Host
  Rat
• Isotype
  IgG, IgG
• Species
  Rat, Chickens, Rats
• Alternative name
  Chicken anti Rat IgG (H + L) secondary antibody (Alk Phos)
• Alternative technique
  antibodies, immunoglobulins
• Alternative to gene target
  anaplastic lymphoma receptor tyrosine kinase, CD246 and NBLST3, ALK and IDBG-42084 and ENSG00000171094 and 238, transferase activity, Plasma membranes, Alk and IDBG-197259 and ENSMUSG00000055471 and 11682, ALK and IDBG-636042 and ENSBTAG00000007379 and 536642

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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