CD8 antibody FITC
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Catalog number61R-CD8cGPFT
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PricePlease ask
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Size100 tests
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Monoclonal Antibodies
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Area of researchImmunology
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Type of ImmunogenCD8 antibody (FITC) was raised in mouse using guinea pig peritoneal T-Cells ..
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Raised inMouse
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SpecificityGuinea Pig
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Cross ReactivityShould be determined by the end user
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IsotypesIgG1
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CloneCT6
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Conjugate TagFITC
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Method of PurificationCD8 antibody (FITC) was purified by Protein A affinity chromatography.
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ConcentrationBatch dependent - please inquire should you have specific requirements
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Form BufferFITC liquid presented in BSA pH7.4 containing 0.09% NaN3.
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StorageStore at 4 deg C for short term storage. Aliquot and store at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
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Shipping conditionsBlue Ice
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Tested forFC
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Usage RecommendationsFC: Neat
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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DescriptionCytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolCD8A
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Short nameCD8 antibody FITC
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative nameMouse monoclonal CD8 antibody (FITC)
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Alternative techniqueantibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameCD8a molecule
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Synonyms gene
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Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data