CD8 antibody FITC
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Catalog number61-001103F
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Price:
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Size50 µg
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CategoryPrimary Antibody
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Antibody SubtypeConjugated Monoclonal Antibodies
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Area of researchImmunology
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Raised inMouse
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SpecificityCD8
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CloneM120409
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Conjugate TagFITC
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Concentration1.0 mg/ml
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StorageStore frozen product at -70 deg C or lower. Avoid repeated freeze/thaw cycles
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Shipping conditionsBlue Ice
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Tested forELISA; WB
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Usage RecommendationsELISA: 1:500-1:1000, WB: 1:500-1:1000
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PropertiesIf you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This fitzgerald Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. fitzgerald FITC is highly sensitive to pH extremes.
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DescriptionCytotoxic T cells cluster differentiators for flow cytometry. This transmembrane glycoprotein is detected by monoclonals and has a lot of clones available with different affinity.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Gene symbolCD8A
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Short nameCD8 antibody FITC
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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LabelFITC
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Alternative nameMouse monoclonal CD8 antibody (FITC)
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Alternative techniqueantibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene nameCD8a molecule
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Synonyms gene
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Synonyms gene name
- CD8 antigen, alpha polypeptide (p32)
- T-cell surface glycoprotein CD8 alpha chain
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Locus
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Discovery year1986-01-01
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data