Biotin reagent Glucose Oxidase
-
Catalog number
65C-CE0203
-
Price
Please ask
-
Size
5 mg
-
-
Category
Proteins
-
Antibody Subtype
Conjugated Proteins
-
Area of research
Miscellaneous
-
Reactivity
N/A
-
Conjugate Tag
Glucose Oxidase
-
Type of protein
Synthetic
-
Method of Purification
Biotin reagent (Glucose Oxidase) was purified by ion exchange chromatography followed by dialysis.
-
Form Buffer
Lyophilized from 0.01M Na3PO4, pH 7.4, with 0.14M NaCl.
-
Storage
Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
-
Shipping conditions
Blue Ice
-
Tested for
ELISA; IHC; WB
-
Usage Recommendations
ELISA: 1:8,000-1:32,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500
-
-
-
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
-
Conjugation
Biotinylated
Similar products