Biotin reagent Glucose Oxidase

  • Catalog number
    65C-CE0203
  • Price
    Please ask
  • Size
    5 mg
  • Category
    Proteins
  • Antibody Subtype
    Conjugated Proteins
  • Area of research
    Miscellaneous
  • Reactivity
    N/A
  • Conjugate Tag
    Glucose Oxidase
  • Type of protein
    Synthetic
  • Method of Purification
    Biotin reagent (Glucose Oxidase) was purified by ion exchange chromatography followed by dialysis.
  • Form Buffer
    Lyophilized from 0.01M Na3PO4, pH 7.4, with 0.14M NaCl.
  • Storage
    Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles.
  • Shipping conditions
    Blue Ice
  • Tested for
    ELISA; IHC; WB
  • Usage Recommendations
    ELISA: 1:8,000-1:32,000, IHC: 1:200-1:1,000, WB: 1:500-1:2,500
  • URL
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Gene target
  • Short name
    reagent Glucose Oxidase
  • Label
    Biotin
  • Alternative name
    Glucose Oxidase conjugated biotin labelling reagent
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