Avidin antibody HRP

Category

Primary Antibody
Antibody Subtype

Conjugated Polyclonal Antibodies
Area of research

Miscellaneous
Type of Immunogen

Avidin antibody (HRP) was raised in rabbit using avidin isolated from hen egg white ..
Raised in

Rabbit
Specificity

Chicken avidin
Cross Reactivity

Should be determined by the end user
Conjugate Tag

HRP
Method of Purification

Avidin antibody (HRP) was purified by ion exchange chromatography followed by dialysis.
Form Buffer

IgG fraction Provided as a liquid in 10 mM Potassium Phosphate and 75 mM NaCl, pH 7.2 with 5mg/ml BSA (IgG and Protease free) and 0.005% Gentamicin Sulfate
Storage

Store at 4 deg C until reconstitution. Following reconstitution aliquot and freeze at -20 deg C for long term storage. Avoid repeated freeze/thaw cycles. Dilute only prior to immediate use.
Shipping conditions

Blue Ice
Tested for

ELISA; IHC; WB
Usage Recommendations

ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000-1:5,000
URL
More info
Properties

If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.
Conjugation

HRP Conjugated
French translation

anticorps

Name

Blotting, Western
Concept

Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data