Aldose reductase protein

• Catalog number
  30R-1234
• Price
  Please ask
• Size
  100 µg

• Category
  Proteins
• Antibody Subtype
  Recombinant Proteins
• Area of research
  Signal Transduction
• Reactivity
  Human
• Residues
  1-316 amino acids: MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF
• Type of protein
  Recombinant
• Expression System
  Escherichia coli
• Grade Purity
  Grater than 95% pure
• Concentration
  1 mg/ml (lot specific)
• Form Buffer
  Provided as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 1 mM DTT, and 10% glycerol.
• Storage
  Store at 4 deg C for short term storage (1/2 weeks). Aliquot and store at -20 deg C or - 70 deg C for long term storage. Avoid repeated freeze/thaw cycles.
• Shipping conditions
  Blue Ice
• Tested for
  SDS-PAGE
• Bioactivity
  Specific activity: approximately 0.5-0.6 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 uM NADPH/min at 25 DEG C.
• Assay Information
  1. Prepare a 1ml reaction mix into a suitable container : The final concentrations are 0.1M sodium phosphate (pH 7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50ul of recombinant AKR1B1 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3. Mix by inversion and Incubate at 25 DEG C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340nm for 3 minutes.
• Additional Information
  Aldose reductase protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
• URL
• More info

• Gene target
  Aldose   reductase   protein  
• Gene symbol
  AKR1B10, MIOX
• Short name
  Aldose reductase protein
• Alternative name
  Purified recombinant Human Aldose reductase protein

Gene info

• Identity
  HGNC:382
• Gene
  AKR1B10
• Long gene name
  aldo-keto reductase family 1 member B10
• Synonyms gene
  • AKR1B11
• Synonyms gene name
  • aldo-keto reductase family 1, member B10 (aldose reductase)
• Synonyms
  • AKR1B12
  • ARL-1
  • HIS
  • ARL1
  • HSI
  • ALDRLn
• Synonyms name
  • aldose reductase-like 1
  • aldo-keto reductase family 1, member B11 (aldose reductase-like)
  • aldose reductase-like peptide
  • aldose reductase-related protein
  • small intestine reductase
• GenBank acession
  • AF052577
• Locus
  7q33
• Discovery year
  2000-08-24
• Entrez gene record
  57016
• Pubmed identfication
  • 9765596
  • 9565553
• RefSeq identity
  • NM_020299
• Classification
  • Aldo-keto reductases
• VEGA ID
  OTTHUMG00000155356

Gene info

• Identity
  HGNC:14522
• Gene
  MIOX
• Long gene name
  myo-inositol oxygenase
• Synonyms gene
  • ALDRL6
• Synonyms gene name
  • aldehyde reductase (aldose reductase) like 6
• Synonyms name
  • kidney-specific protein 32
• GenBank acession
  • AF197129
• Locus
  22q13.33
• Discovery year
  2002-02-06
• Entrez gene record
  55586
• Pubmed identfication
  • 10944187
  • 11716759
• RefSeq identity
  • NM_017584
• VEGA ID
  OTTHUMG00000150207

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