Aldose Reductase Activity Kit (Colorimetric)

• Catalog number
  K369-100
• Price
  Please ask
• Size
  100 assays

• Description
  Aldose Reductase Activity Assay
• Summary
  Aldose Reductase Activity Kit is a simple, sensitive assay to study Aldose Reductase activity in a variety of samples.
• Detection Method
  Colorimetric assay at 340 nm
• Species Reactivity
  All, Mammalian
• Applications
  Analysis of polyol pathway, Measurement of Aldose Reductase activity in various tissues/cells
• Sample Type
  Purified enzymes, Cell culture: HepG2 cells, etc., Tissue homogenates: Kidney, Spleen, etc.
• Features Benefits
  Can detect as little as 0.1 mU of Aldose Reductase activity in a variety of samples., Simple Procedure, Sensitive Assay to measuring Aldose Reductase activity
• Storage Conditions
  -20°C
• Shipping Conditions
  Gel Pack
• Shelf life
  12 months
• Background
  Aldose Reductase (Aldehyde Reductase; AR; ALR2; EC 1.1.1.21), a member of the aldo-keto reductase superfamily, catalyzes the NADPH-dependent reduction of a wide variety of aldoses (molecules containing carbonyl groups) to their corresponding alcohols. It is a key enzyme in the polyol (polyhydric alcohol) pathway and has been implicated to play a critical role in diabetes and cardiovascular complications. Aldose Reductase is the key factor in the reduction of glucose to sorbitol and its activity is higher during hyperglycemic conditions. The synthesis and accumulation of sorbitol due to AR activity is the main cause of diabetic complications, such as cataracts, retinopathy, neuropathy and nephropathy. Thus, AR could be used as a therapeutic target for the clinical treatment of secondary complications of diabetes. BioVision’s Aldose Reductase Activity Kit is based on the ability of AR to catalyze the oxidation of NADPH. The reaction progress is followed by monitoring the decrease in absorbance at 340 nm. The assay has been optimized to be monitored using a 96 well plate. The assay is simple, sensitive and can detect as little as 0.1 mU of Aldose Reductase activity in a variety of samples.
• Data Sheet Link
• Properties
  Colorimetric assays or detection use UV absorption or enzymatic color reaction.

• Gene target
  Aldose   Reductase   Activity   Kit  
• Short name
  Aldose Reductase Activity Kit (Colorimetric)
• Technique
  colorimetric, colorimetry
• Alternative name
  Aldose Reductase Activity reagent (colorimetry)
• Alternative technique
  assays, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

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