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Long name
Rabbit Polyclonal Antibody against Mouse NTAL / LAB, Clone: PAb
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Antigen
NTAL / LAB
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Species reactivity
Mouse (Mus musculus), Rat, Other species Not tested
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Negative species reactivity
Human
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Clonality
Polyclonal Antibody (PAb)
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Clone
Polyclonal
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Applications
WB, ICC
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Immunogen
Recombinant mouse NTAL (aa 30-203).
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Format conjugation
purified; unconjugated
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Specificity
The polyclonal antibody reacts with Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 25 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
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Research area
Adaptor Proteins (Rodent), Adaptor Proteins
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Concentration
1 mg/ml
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Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storage
Rabbit Polyclonal Antibody against Mouse NTAL / LAB, Clone: PAb is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified from rabbit serum by affinity chromatography
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References
*Lebduska P, Korb J, Tůmová M, Heneberg P, Dráber P: Topography of signaling molecules as detected by electron microscopy on plasma_x000D_ membrane sheets isolated from non-adherent mast cells. J Immunol Methods. 2007 Dec 1;328(1-2):139-51._x000D_ , *Volná P, Lebduska P, Dráberová L, Símová S, Heneberg P, Boubelík M, Bugajev V, Malissen B, Wilson BS, Horejsí V, Malissen M, Dráber P.: Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med. 2004 Oct 18;200(8):1001-13., *Heneberg P, Lebduska P, Draberova L, Korb J, Draber P.: Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol. 2006 Oct;36(10):2795-806. _x000D_
