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Long name
Mouse Monoclonal Antibody against Human Kappa light chains, Clone: TB28-2
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Antigen
Kappa light chains
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Species reactivity
Human (Homo sapiens)
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
TB28-2
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Applications
FC
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Immunogen
Human IgG-kappa myeloma protein
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Format conjugation
APC
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Specificity
The antibody TB28-2 recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains.
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Research area
Immunoglobulins (Human)
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Storage buffer
The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
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Shipping and storage
Mouse Monoclonal Antibody against Human Kappa light chains, Clone: TB28-2 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda; both having a molecular weight of 22.5kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by affinity chromatography
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References
*Jourdan M, Caraux A, De Vos J, Fiol G, Larroque M, Cognot C, Bret C, Duperray C, Hose D, Klein B: An in vitro model of differentiation of memory B cells into plasmablasts and_x000D_ plasma cells including detailed phenotypic and molecular characterization. Blood. 2009 Dec 10;114(25):5173-81., *Böttcher S, Ritgen M, Buske S, Gesk S, Klapper W, Hoster E, Hiddemann W, Unterhalt M, Dreyling M, Siebert R, Kneba M, Pott C; EU MCL MRD Group: Minimal residual disease detection in mantle cell lymphoma: methods and_x000D_ significance of four-color flow cytometry compared to consensus IGH-polymerase chain reaction at initial staging and for follow-up examinations. Haematologica. 2008 Apr;93(4):551-9., *Nakamura T, Kubagawa H, Cooper MD: Heterogeneity of immunoglobulin-associated molecules on human B cells identified by monoclonal antibodies. Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8522-6., *Kiyotaki M, Cooper MD, Bertoli LF, Kearney JF, Kubagawa H: Monoclonal anti-Id antibodies react with varying proportions of human B lineage cells. J Immunol. 1987 Jun 15;138(12):4150-8., *Karandikar NJ, Aquino DB, McKenna RW, Kroft SH: Transient myeloproliferative disorder and acute myeloid leukemia in Down syndrome. An immunophenotypic analysis. Am J Clin Pathol. 2001 Aug;116(2):204-10.
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
