-
Long name
Rabbit Polyclonal Antibody against Recognizes fusion proteins in all species GFP / Green Fluorescent Protein, Clone: PAb
-
Antigen
GFP
-
Species reactivity
Recognizes fusion proteins in all species
-
Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
-
Clonality
Polyclonal Antibody (PAb)
-
Clone
Polyclonal
-
Applications
IP, WB, ICC
-
Immunogen
EGFP, a native full-length protein_x000D_
-
Format conjugation
purified; unconjugated
-
Specificity
The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species._x000D_
-
Research area
Epitope Tags
-
Concentration
1 mg/ml
-
Storage buffer
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
-
Shipping and storage
Rabbit Polyclonal Antibody against Recognizes fusion proteins in all species GFP / Green Fluorescent Protein, Clone: PAb is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
-
Background
Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (λ=509 nm) when excited (excitation by Blue or UV light, absorption peak λ=395 nm)._x000D_ GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein._x000D_ _x000D_ GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols._x000D_ _x000D_ To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties._x000D_ _x000D_ Antibodies raised against full-length GFP variants should also detect other variants of the protein.
-
Purity
> 95% (by SDS-PAGE)
-
Purification method
Purified from rabbit serum by affinity chromatography
-
-
References
*Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25(11):2326-37. Epub 2006 May 25.
-
Description
Fluorescent microspheres, beads and particles applications including blood flow determination, tracing, fluorimetry, in vivo imaging and calibration of imaging and flow cytometry instruments. Because our fluorescent dyes are incorporated in the bead and not just on the surface, they are relatively immune to photo bleaching and other environmental factors. Spheres are in difference sizes and fluorescences, high intensity, FITC, GFP, red, green, yellow, light yellow, sky blue, blue, orange, deep-red, Nile-red, purple, mcherry. The diameter of the spheres is usually higher than 50nm and in the micrometer um range.
