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GFP / Green Fluorescent Protein PAb

  • Catalog number
    11-476-C100
  • Product name
    GFP / Green Fluorescent Protein PAb
  • Size
    100ug
  • Short name
    Anti-GFP / Green Fluorescent Protein PAb
  • Long name
    Rabbit Polyclonal Antibody against Recognizes fusion proteins in all species GFP / Green Fluorescent Protein, Clone: PAb
  • Antigen
    GFP
  • Host
    Rabbit (Oryctolagus cuniculus)
  • Species reactivity
    Recognizes fusion proteins in all species
  • Negative species reactivity
    at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
  • Clonality
    Polyclonal Antibody (PAb)
  • Clone
    Polyclonal
  • Isotype
    IgG (various subtypes)
  • Applications
    IP, WB, ICC
  • Immunogen
    EGFP, a native full-length protein_x000D_
  • Format conjugation
    purified; unconjugated
  • Specificity
    The polyclonal antibody recognizes GFP, EGFP, EYFP fusion proteins in all species._x000D_
  • Research area
    Epitope Tags
  • Concentration
    1 mg/ml
  • Storage buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  • Shipping and storage
    Rabbit Polyclonal Antibody against Recognizes fusion proteins in all species GFP / Green Fluorescent Protein, Clone: PAb is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
  • Background
    Green fluorescence protein (GFP) is a 27 KDa protein derived from the bioluminiscent jellyfish Aquorea victoria, emiting green light (λ=509 nm) when excited (excitation by Blue or UV light, absorption peak λ=395 nm)._x000D_ GFP is a useful tool in cell biology research, as its intrinsic fluorescence can be visualized in living cells. Light-stimulated GFP fluorescence is species-independent and a fluorescence has been reported from many different types of GFP-expressing hosts, including microbes, invertebrates, vertebrates and plants. No exogenous substrates and cofactors are required for the fluorescence of GFP, since GFP autocatalytically forms a fluorescent pigment from natural amino acids present in the nascent protein._x000D_ _x000D_ GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP is widely used as a reporter (tag) for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without any further staining. Other applications of GFP include measurement of distance between proteins through fluorescence energy transfer (FRET) protocols._x000D_ _x000D_ To increase a fluorescence intensity of GFP, chomophore mutations have been created. The EnhancedGFP has a fluorescence 35 times more intense than the wt-GFP. Mutagenesis of GFP has produced also many mutants (e.g. Yellow Fluorescent Protein, Cyan Fluorescent Protein) with warying spectral properties._x000D_ _x000D_ Antibodies raised against full-length GFP variants should also detect other variants of the protein.
  • Purity
    > 95% (by SDS-PAGE)
  • Purification method
    Purified from rabbit serum by affinity chromatography
  • Datasheet
    Datasheet
  • References
    *Valenta T, Lukas J, Doubravska L, Fafilek B, Korinek V: HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies. EMBO J. 2006 Jun 7;25(11):2326-37. Epub 2006 May 25.
  • Gene target
    GFP / Protein PAb
  • isotype filter
    • IgG various subtypes
  • Label
    gfp
  • label filter
    • gfp