CD54 / ICAM-1 monoclonal antibody
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Catalog number11-228-C100
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PricePlease ask
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Size100ug
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Long nameMouse Monoclonal Antibody against Rat CD54 / ICAM-1, Clone: MEM-111
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AntigenCD54
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Species reactivityRat, Human (Homo sapiens), Bovine
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Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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ClonalityMonoclonal Antibody (Mab)
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CloneMEM-111
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ApplicationsFC, WB, IHC(P), ELISA
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ImmunogenRaji human Burkitt's lymphoma cell line
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Format conjugationpurified; unconjugated
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SpecificityThe antibody MEM-111 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.HLDA VI; WS Code AS A049
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Research areaCD Antigens & MHC (Veterinary), CD and Related Antigens & MHC (Rodent), CD and Related Antigens (Human)
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Concentration1 mg/ml
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Storage bufferPhosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
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Shipping and storageMouse Monoclonal Antibody against Rat CD54 / ICAM-1, Clone: MEM-111 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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BackgroundCD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. _x000D_
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Purity> 95% (by SDS-PAGE)
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Purification methodPurified by protein-A affinity chromatography
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References*Bacáková L, Mares V, Lysá V, Svorcík V: Molecular mechanisms of improved adhesion and growth of an endothelial cell line cultured on polystyrene implanted with fluorine ions. Biomaterials. 2000 Jun;21(11):1173-9._x000D_ _x000D_ _x000D_ , *Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J Immunol. 2007 Dec 15;179(12):8128-36., *Bacáková L, Mares V, Bottone MG, Pellicciari C, Lisá V, Svorcík V: Fluorine ion-implanted polystyrene improves growth and viability of vascular smooth muscle cells in culture. J Biomed Mater Res. 2000 Mar 5;49(3):369-79._x000D_ _x000D_ , *Leeuwenberg JFM et al.: E-selectin and intercellular adhesion molecule-1 are released by activated human endothelial cells in vitro. Immunology 77, 543 (1992)._x000D_ , *Tachimoto H. et al., Eotaxin-2 Alters Eosinophil Integrin Function via Mitogen-Activated Protein Kinases. Am J Respir Cell Mol Biol 26, 645 (2002)., *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., *Hertel L et al.: Susceptibility of Immature and Mature Langerhans Cell-Type Dendritic Cells to Infection and Immunomodulation by Human Cytomegalovirus. J Virol. 77, 7563 (2003). , *Nagaraju K et al.: A variety of cytokines and immunologically relevant surface molecules are expressed by normal human skeletal muscle cells under proinflammatory stimuli. Clin Exp Immunol 113, 407 (1998)., *Burdick MM et al.: Colon carcinoma cell glycolipids, integrins, and other glycoproteins mediate adhesion to HUVECs under flow. Am J Physiol Cell Physiol 284, C977 (2003).
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PropertiesIf you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translationanticorps
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Gene target
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Gene symbolICAM1, ICAM3, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1, MIR16-1
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Short nameAnti-CD54 / ICAM-1 Mab
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TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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HostMouse (Mus musculus)
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IsotypeIgG2a
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Alternative nameMouse Monoclonal to CD54 / ICAM-1
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameintercellular adhesion molecule 1
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Synonyms
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Synonyms name
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Locus
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Discovery year1989-04-24
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Entrez gene record
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Pubmed identfication
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Classification
- Ig-like cell adhesion molecule family
- Immunoglobulin like domain containing
- Receptor ligands
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameintercellular adhesion molecule 3
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Synonyms
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Locus
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Discovery year1994-05-19
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Entrez gene record
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Pubmed identfication
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Classification
- Ig-like cell adhesion molecule family
- Immunoglobulin like domain containing
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin heavy variable (II)-1-1 (pseudogene)
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Synonyms gene name
- immunoglobulin heavy variable (II)-1-1
- immunoglobulin heavy variable (II)-1-1 pseudogene
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-17
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Entrez gene record
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RefSeq identity
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Classification
- Immunoglobulin heavy locus at 14q32.33
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1/206 family
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 8-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 8-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nametRNA-undetermined (NNN) 7-1
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Synonyms gene name
- transfer RNA-undetermined (NNN) 7-1
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GenBank acession
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Locus
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Discovery year2014-06-20
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Entrez gene record
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Pubmed identfication
Gene info
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Identity
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Gene
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Long gene nameimmunoglobulin kappa variable 1/OR2-1 (pseudogene)
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Synonyms gene
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Synonyms gene name
- immunoglobulin kappa variable 1/OR-1
- immunoglobulin kappa variable 1/OR-1 pseudogene
- immunoglobulin kappa variable 1/OR-1 (pseudogene)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-04-18
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Entrez gene record
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Pubmed identfication
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Classification
- Immunoglobulin kappa (IGK) orphons
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namemicroRNA 101-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR101 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 1289-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2008-11-03
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR1289 family
Gene info
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Identity
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Gene
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Long gene namemicroRNA 16-1
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Synonyms gene
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Synonyms
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Locus
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Discovery year2004-04-23
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Entrez gene record
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RefSeq identity
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Classification
- MicroRNA MIR15/16 family
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
