CD54 monoclonal antibody

• Catalog number
  12-625-C100
• Price
  Please ask
• Size
  100ug

• Long name
  Rat Monoclonal Antibody against Mouse CD54, Clone: YN1/1.7.4
• Antigen
  CD54 (mouse)
• Species reactivity
  Mouse (Mus musculus)
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  YN1/1.7.4
• Applications
  FC, IP, WB, IHC(F), ICC, FUNC
• Immunogen
  Mouse NS-1 cells_x000D_
• Format conjugation
  low endotoxin, unconjugated
• Specificity
  The rat monoclonal antibody YN1/1.7.4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation. _x000D_
• Research area
  CD and Related Antigens & MHC (Rodent)
• Concentration
  1 mg/ml
• Storage buffer
  Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
• Shipping and storage
  Rat Monoclonal Antibody against Mouse CD54, Clone: YN1/1.7.4 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  CD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. _x000D_
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by protein-G affinity chromatography
• Datasheet
• References
  _x000D_ , *Chin JE, Winterrowd GE, Hatfield CA, Brashler JR, Griffin RL, Vonderfecht SL, Kolbasa KP, Fidler SF, Shull KL, Krzesicki RF, Ready KA, Dunn CJ, Sly LM, Staite ND, Richards IM: Involvement of intercellular adhesion molecule-1 in the antigen-induced infiltration of eosinophils and lymphocytes into the airways in a murine model of pulmonary inflammation. Am J Respir Cell Mol Biol. 1998 Feb;18(2):158-67._x000D_ , *Salas A, Gironella M, Salas A, Soriano A, Sans M, Iovanna J, Piqué JM, Panés J: Nitric oxide supplementation ameliorates dextran sulfate sodium-induced colitis in mice. Lab Invest. 2002 May;82(5):597-607._x000D_ , *Sasaki M, Elrod JW, Jordan P, Itoh M, Joh T, Minagar A, Alexander JS: CYP450 dietary inhibitors attenuate TNF-alpha-stimulated endothelial molecule expression and leukocyte adhesion. Am J Physiol Cell Physiol. 2004 Apr;286(4):C931-9._x000D_ , *Sasaki M, Ostanin D, Elrod JW, Oshima T, Jordan P, Itoh M, Joh T, Minagar A, Alexander JS: TNF-alpha -induced endothelial cell adhesion molecule expression is cytochrome P-450 monooxygenase dependent. Am J Physiol Cell Physiol. 2003 Feb;284(2):C422-8._x000D_ , *Ma Z, Sharp KA, Janmey PA, Finkel TH: Surface-anchored monomeric agonist pMHCs alone trigger TCR with high sensitivity. PLoS Biol. 2008 Feb;6(2):e43._x000D_ , *Greicius G, Westerberg L, Davey EJ, Buentke E, Scheynius A, Thyberg J, Severinson E: Microvilli structures on B lymphocytes: inducible functional domains? Int Immunol. 2004 Feb;16(2):353-64._x000D_ , *Barrett SP, Riordon A, Toh BH, Gleeson PA, van Driel IR: Homing and adhesion molecules in autoimmune gastritis. J Leukoc Biol. 2000 Feb;67(2):169-73_x000D_ , *Burns AR, Takei F, Doerschuk CM: Quantitation of ICAM-1 expression in mouse lung during pneumonia. J Immunol. 1994 Oct 1;153(7):3189-98._x000D_ , *Horley KJ, Carpenito C, Baker B, Takei F: Molecular cloning of murine intercellular adhesion molecule (ICAM-1). EMBO J. 1989 Oct;8(10):2889-96._x000D_ , *Soriano A, Salas A, Salas A, Sans M, Gironella M, Elena M, Anderson DC, Piqué JM, Panés J: VCAM-1, but not ICAM-1 or MAdCAM-1, immunoblockade ameliorates DSS-induced colitis in mice. Lab Invest. 2000 Oct;80(10):1541-51._x000D_
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  CD54   monoclonal  
• Gene symbol
  ICAM1
• Short name
  Anti-CD54 Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Rat (Rattus norvegicus)
• Isotype
  IgG2b
• Alternative name
  Rat Monoclonal to CD54 (mouse)
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:5344
• Gene
  ICAM1
• Long gene name
  intercellular adhesion molecule 1
• Synonyms
  • BB2
  • CD54
• Synonyms name
  • human rhinovirus receptor
• Locus
  19p13.2
• Discovery year
  1989-04-24
• Entrez gene record
  3383
• Pubmed identfication
  • 2453850
  • 3871395
• Classification
  • Ig-like cell adhesion molecule family
  • Immunoglobulin like domain containing
  • Receptor ligands
  • CD molecules
• VEGA ID
  OTTHUMG00000180403

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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