- Catalog number12-625-C100
- Product nameCD54 monoclonal antibody
- Size100ug
- PriceAsk For Price
- Short nameAnti-CD54 Mab
- Long nameRat Monoclonal Antibody against Mouse CD54, Clone: YN1/1.7.4
- AntigenCD54 (mouse)
- HostRat (Rattus norvegicus)
- Species reactivityMouse (Mus musculus)
- Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
- ClonalityMonoclonal Antibody (Mab)
- CloneYN1/1.7.4
- IsotypeIgG2b
- ApplicationsFC, IP, WB, IHC(F), ICC, FUNC
- ImmunogenMouse NS-1 cells_x000D_
- Format conjugationlow endotoxin, unconjugated
- SpecificityThe rat monoclonal antibody YN1/1.7.4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation. _x000D_
- Research areaCD and Related Antigens & MHC (Rodent)
- Concentration1 mg/ml
- Storage bufferAzide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
- Shipping and storageRat Monoclonal Antibody against Mouse CD54, Clone: YN1/1.7.4 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
- BackgroundCD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation. _x000D_
- Purity> 95% (by SDS-PAGE)
- Purification methodPurified by protein-G affinity chromatography
- Datasheet
- References_x000D_ , *Chin JE, Winterrowd GE, Hatfield CA, Brashler JR, Griffin RL, Vonderfecht SL, Kolbasa KP, Fidler SF, Shull KL, Krzesicki RF, Ready KA, Dunn CJ, Sly LM, Staite ND, Richards IM: Involvement of intercellular adhesion molecule-1 in the antigen-induced infiltration of eosinophils and lymphocytes into the airways in a murine model of pulmonary inflammation. Am J Respir Cell Mol Biol. 1998 Feb;18(2):158-67._x000D_ , *Salas A, Gironella M, Salas A, Soriano A, Sans M, Iovanna J, Piqué JM, Panés J: Nitric oxide supplementation ameliorates dextran sulfate sodium-induced colitis in mice. Lab Invest. 2002 May;82(5):597-607._x000D_ , *Sasaki M, Elrod JW, Jordan P, Itoh M, Joh T, Minagar A, Alexander JS: CYP450 dietary inhibitors attenuate TNF-alpha-stimulated endothelial molecule expression and leukocyte adhesion. Am J Physiol Cell Physiol. 2004 Apr;286(4):C931-9._x000D_ , *Sasaki M, Ostanin D, Elrod JW, Oshima T, Jordan P, Itoh M, Joh T, Minagar A, Alexander JS: TNF-alpha -induced endothelial cell adhesion molecule expression is cytochrome P-450 monooxygenase dependent. Am J Physiol Cell Physiol. 2003 Feb;284(2):C422-8._x000D_ , *Ma Z, Sharp KA, Janmey PA, Finkel TH: Surface-anchored monomeric agonist pMHCs alone trigger TCR with high sensitivity. PLoS Biol. 2008 Feb;6(2):e43._x000D_ , *Greicius G, Westerberg L, Davey EJ, Buentke E, Scheynius A, Thyberg J, Severinson E: Microvilli structures on B lymphocytes: inducible functional domains? Int Immunol. 2004 Feb;16(2):353-64._x000D_ , *Barrett SP, Riordon A, Toh BH, Gleeson PA, van Driel IR: Homing and adhesion molecules in autoimmune gastritis. J Leukoc Biol. 2000 Feb;67(2):169-73_x000D_ , *Burns AR, Takei F, Doerschuk CM: Quantitation of ICAM-1 expression in mouse lung during pneumonia. J Immunol. 1994 Oct 1;153(7):3189-98._x000D_ , *Horley KJ, Carpenito C, Baker B, Takei F: Molecular cloning of murine intercellular adhesion molecule (ICAM-1). EMBO J. 1989 Oct;8(10):2889-96._x000D_ , *Soriano A, Salas A, Salas A, Sans M, Gironella M, Elena M, Anderson DC, Piqué JM, Panés J: VCAM-1, but not ICAM-1 or MAdCAM-1, immunoblockade ameliorates DSS-induced colitis in mice. Lab Invest. 2000 Oct;80(10):1541-51._x000D_
- Gene targetCD54 monoclonal
- Identity:HGNC:5344
- Gene:ICAM1
- Long gene name:intercellular adhesion molecule 1
- Synonyms:BB2CD54
- Discovery year:1989-04-24
- Entrez gene record:3383
- Pubmed identification:2453850, 3871395
- Classification:Ig-like cell adhesion molecule family, Immunoglobulin like domain containing, Receptor ligands, CD molecules
- VEGA ID:OTTHUMG00000180403
- Gene symbolICAM1
- isotype filter
- IgG2b
- technique filter
- Antibody
- TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
- Alternative techniqueantibodies