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Long name
Mouse Monoclonal Antibody against Human CD47 / Integrin Associated Protein, Clone: MEM-122
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Antigen
CD47
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Species reactivity
Non-Human (Homo sapiens) Primates, Human (Homo sapiens), Porcine
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Negative species reactivity
at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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Clonality
Monoclonal Antibody (Mab)
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Clone
MEM-122
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Applications
FC, WB, IHC(F)
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Immunogen
COS-7 (African green monkey) cells
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Format conjugation
Pacific Blue
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Specificity
The antibody MEM-122 reacts with CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines._x000D_ HLDA VI; WS Code AS A051
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Research area
CD Antigens & MHC (Veterinary), CD and Related Antigens (Human)
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Storage buffer
The reagent is provided in stabilizing Tris buffered saline (TBS) solution containing 15 mM sodium azide.
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Shipping and storage
Mouse Monoclonal Antibody against Human CD47 / Integrin Associated Protein, Clone: MEM-122 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by affinity chromatography
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References
*Smith RE, Patel V, Seatter SD, Deehan MR, Brown MH, Brooke GP, Goodridge HS, Howard CJ, Rigley KP, Harnett W, Harnett MM: A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes. Blood. 2003 Oct 1;102(7):2532-40., *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps