CD47 / Integrin Associated Protein monoclonal antibody
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Catalog number11-225-C025
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Price:
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Size25ug
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Long nameMouse Monoclonal Antibody against Human CD47 / Integrin Associated Protein, Clone: MEM-122
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AntigenCD47
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Species reactivityNon-Human (Homo sapiens) Primates, Human (Homo sapiens), Porcine
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Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
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ClonalityMonoclonal Antibody (Mab)
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CloneMEM-122
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ApplicationsFC, WB, IHC(F)
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ImmunogenCOS-7 (African green monkey) cells
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Format conjugationpurified; unconjugated
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SpecificityThe antibody MEM-122 reacts with CD47 (Integrin Associated Protein), a 50-55 kDa membrane adhesion molecule (thrombospondin receptor; immunoglobulin supergene family) expressed on leukocytes, platelets and erythrocytes. It is also expressed on epithelial cells, endothelial cells, fibroblasts and many tumor cell lines._x000D_ HLDA VI; WS Code AS A051
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Research areaCD Antigens & MHC (Veterinary), CD and Related Antigens (Human)
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Concentration1 mg/ml
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Storage bufferTris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
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Shipping and storageMouse Monoclonal Antibody against Human CD47 / Integrin Associated Protein, Clone: MEM-122 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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BackgroundCD47 (integrin-associated protein, IAP) is an ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Binding secreted thrombospondin-1, CD47 counteracts graft vascularization. CD47 acts also as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion and T cell activation. CD47 is also involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
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Purity> 95% (by SDS-PAGE)
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Purification methodPurified by precipitation and chromatography
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References*Smith RE, Patel V, Seatter SD, Deehan MR, Brown MH, Brooke GP, Goodridge HS, Howard CJ, Rigley KP, Harnett W, Harnett MM: A novel MyD-1 (SIRP-1alpha) signaling pathway that inhibits LPS-induced TNFalpha production by monocytes. Blood. 2003 Oct 1;102(7):2532-40., *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
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PropertiesIf you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translationanticorps
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Gene target
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Gene symbolCD47
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Short nameAnti-CD47 / Integrin Associated Protein Mab
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TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
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HostMouse (Mus musculus)
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IsotypeIgM
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Alternative nameMouse Monoclonal to CD47 / Integrin Associated Protein
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Alternative techniqueantibodies
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Alternative to gene targetCD47 molecule, IAP and MER6 and OA3, CD47 and IDBG-48744 and ENSG00000196776 and 961, thrombospondin receptor activity, Plasma membranes, Cd47 and IDBG-165137 and ENSMUSG00000055447 and 16423, CD47 and IDBG-631689 and ENSBTAG00000003585 and 282661
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Gene info
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Identity
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Gene
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Long gene nameCD47 molecule
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Synonyms gene
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Synonyms gene name
- CD47 antigen (Rh-related antigen, integrin-associated signal transducer)
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Synonyms
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Synonyms name
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Locus
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Discovery year1994-12-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- CD molecules
- Ig-like cell adhesion molecule family
- Immunoglobulin like domain containing
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VEGA ID