CD33 monoclonal antibody

• Catalog number
  12-662-C100
• Price
  Please ask
• Size
  100ug

• Long name
  Mouse Monoclonal Antibody against Human CD33, Clone: WM53
• Antigen
  CD33
• Species reactivity
  Human (Homo sapiens), Non-Human (Homo sapiens) Primates
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  WM53
• Applications
  FC, IP, WB, IHC(F), ICC, MC, FUNC
• Immunogen
  Human AML cells
• Format conjugation
  low endotoxin, unconjugated
• Specificity
  The mouse monoclonal antibody WM53 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.HLDA IV; WS Code M-505
• Research area
  CD and Related Antigens (Human)
• Concentration
  1 mg/ml
• Storage buffer
  Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
• Shipping and storage
  Mouse Monoclonal Antibody against Human CD33, Clone: WM53 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by protein-A affinity chromatography
• Datasheet
• References
  Contact us for the most recent list of references
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  CD33   monoclonal  
• Gene symbol
  CD33
• Short name
  Anti-CD33 Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  IgG1
• Alternative name
  Mouse Monoclonal to CD33
• Alternative technique
  antibodies
• Alternative to gene target
  CD33 molecule, p67 and SIGLEC-3 and SIGLEC3, CD33 and IDBG-65737 and ENSG00000105383 and 945, protein binding, Cell surfaces

Gene info

• Identity
  HGNC:1659
• Gene
  CD33
• Long gene name
  CD33 molecule
• Synonyms gene name
  • CD33 antigen (gp67)
• Synonyms
  • SIGLEC3
  • SIGLEC-3
  • p67
  • FLJ00391
• Synonyms name
  • sialic acid binding Ig-like lectin 3
• GenBank acession
  • M23197
• Locus
  19q13.41
• Discovery year
  1986-01-01
• Entrez gene record
  945
• Pubmed identfication
  • 3139766
  • 9465907
• RefSeq identity
  • NM_001772
• Classification
  • CD molecules
  • Sialic acid binding Ig like lectins
  • V-set domain containing
• VEGA ID
  OTTHUMG00000182891

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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