CD33 monoclonal antibody

• Catalog number
  PB-365-T100
• Price
  Please ask
• Size
  100 assays

• Long name
  Mouse Monoclonal Antibody against Human CD33, Clone: HIM3-4
• Antigen
  CD33
• Species reactivity
  Human (Homo sapiens), Non-Human (Homo sapiens) Primates
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  HIM3-4
• Applications
  FC
• Immunogen
  NFMY-9s human cell line
• Format conjugation
  Pacific Blue
• Specificity
  The antibody HIM3-4 reacts with CD33, a 67 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells; it is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.HLDA V; WS Code M MA112HLDA VI; WS Code M MA47
• Research area
  CD Antigens & MHC (Veterinary), CD and Related Antigens (Human)
• Storage buffer
  The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
• Shipping and storage
  Mouse Monoclonal Antibody against Human CD33, Clone: HIM3-4 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia.
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by affinity chromatography
• Datasheet
• References
  *McCormack E, Mujic M, Osdal T, Bruserud O, Gjertsen BT: Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia. Blood. 2013 Feb 14;121(7):e34-42. doi: 10.1182/blood-2012-05-429555., *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997)., *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995).
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  CD33   monoclonal  
• Gene symbol
  CD33
• Short name
  Anti-CD33 Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Mouse (Mus musculus)
• Isotype
  IgG1
• Alternative name
  Mouse Monoclonal to CD33
• Alternative technique
  antibodies
• Alternative to gene target
  CD33 molecule, p67 and SIGLEC-3 and SIGLEC3, CD33 and IDBG-65737 and ENSG00000105383 and 945, protein binding, Cell surfaces

Gene info

• Identity
  HGNC:1659
• Gene
  CD33
• Long gene name
  CD33 molecule
• Synonyms gene name
  • CD33 antigen (gp67)
• Synonyms
  • SIGLEC3
  • SIGLEC-3
  • p67
  • FLJ00391
• Synonyms name
  • sialic acid binding Ig-like lectin 3
• GenBank acession
  • M23197
• Locus
  19q13.41
• Discovery year
  1986-01-01
• Entrez gene record
  945
• Pubmed identfication
  • 3139766
  • 9465907
• RefSeq identity
  • NM_001772
• Classification
  • CD molecules
  • Sialic acid binding Ig like lectins
  • V-set domain containing
• VEGA ID
  OTTHUMG00000182891

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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