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Long name
Rat Monoclonal Antibody against Mouse CD18 Integrin beta2 subunit, Clone: M18/2
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Antigen
CD18 (mouse)
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Species reactivity
Mouse (Mus musculus)
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Negative species reactivity
Canine (Dog)
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Clonality
Monoclonal Antibody (Mab)
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Clone
M18/2
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Applications
FC, IP, WB, IHC(P), IHC(F), FUNC
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Immunogen
Murine cytotoxic T cell glycoproteins_x000D_
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Format conjugation
low endotoxin, unconjugated
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Specificity
The rat monoclonal antibody M18/2 recognizes CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes._x000D_
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Research area
CD and Related Antigens & MHC (Rodent)
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Concentration
1 mg/ml
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Storage buffer
Azide free phosphate buffered saline (PBS), approx. pH 7.4; 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
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Shipping and storage
Rat Monoclonal Antibody against Mouse CD18 Integrin beta2 subunit, Clone: M18/2 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. _x000D_
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by protein-G affinity chromatography
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References
_x000D_ , *Abraham C, Miller J: Molecular mechanisms of IL-2 gene regulation following costimulation through LFA-1. J Immunol. 2001 Nov 1;167(9):5193-201._x000D_ , *Varga G, Balkow S, Wild MK, Stadtbaeumer A, Krummen M, Rothoeft T, Higuchi T, Beissert S, Wethmar K, Scharffetter-Kochanek K, Vestweber D, Grabbe S: Active MAC-1 (CD11b/CD18) on DCs inhibits full T-cell activation. Blood. 2007 Jan 15;109(2):661-9. _x000D_ , *Barlow SC, Langston W, Matthews KM, Chidlow JH Jr, Kevil CG: CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes. Am J Pathol. 2004 Dec;165(6):1849-52._x000D_ , *Cullere X, Lauterbach M, Tsuboi N, Mayadas TN: Neutrophil-selective CD18 silencing using RNA interference in vivo. Blood. 2008 Apr 1;111(7):3591-8._x000D_ , *Sakurai E, Taguchi H, Anand A, Ambati BK, Gragoudas ES, Miller JW, Adamis AP, Ambati J: Targeted disruption of the CD18 or ICAM-1 gene inhibits choroidal neovascularization. Invest Ophthalmol Vis Sci. 2003 Jun;44(6):2743-9._x000D_ , *Avni O, Pur Z, Yefenof E, Baniyash M: Complement receptor 3 of macrophages is associated with galectin-1-like protein. J Immunol. 1998 Jun 15;160(12):6151-8._x000D_ , *Watts GM, Beurskens FJ, Martin-Padura I, Ballantyne CM, Klickstein LB, Brenner MB, Lee DM: Manifestations of inflammatory arthritis are critically dependent on LFA-1. J Immunol. 2005 Mar 15;174(6):3668-75._x000D_ , *Zahalka MA, Naor D: Beta 2-integrin dependent aggregate formation between LB T cell lymphoma and spleen cells: assessment of correlation with spleen invasiveness. Int Immunol. 1994 Jun;6(6):917-24._x000D_ , *Blocking lymphoma invasiveness with a monoclonal antibody directed against the beta-chain of the leukocyte adhesion molecule (CD18). J Immunol. 1993 May 15;150(10):4466-77._x000D_ , *Sanchez-Madrid F, Simon P, Thompson S, Springer TA: Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of_x000D_ two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983 Aug 1;158(2):586-602._x000D_
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
