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Long name
Mouse Monoclonal Antibody against Human CD18 / Integrin beta2 subunit, Clone: MEM-148
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Antigen
CD18
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Species reactivity
Human (Homo sapiens)
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Negative species reactivity
Porcine
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Clonality
Monoclonal Antibody (Mab)
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Clone
MEM-148
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Applications
FC, IP, WB, FUNC
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Immunogen
Peripheral blood mononuclear cells
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Format conjugation
PE
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Specificity
The antibody MEM-148 recognizes an epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit; beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes._x000D_ HLDA VI; WS Code AS A052
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Research area
CD and Related Antigens (Human)
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Storage buffer
The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
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Shipping and storage
Mouse Monoclonal Antibody against Human CD18 / Integrin beta2 subunit, Clone: MEM-148 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
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Background
CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
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Purity
> 95% (by SDS-PAGE)
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Purification method
Purified by affinity chromatography
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References
*Osicka R, Osickova A, Hasan S, Bumba L, Cerny J, Sebo P: Bordetella adenylate cyclase toxin is a unique ligand of the integrin complement receptor 3. Elife. 2015 Dec 9;4:e10766., *Kanderova V, Kuzilkova D, Stuchly J, Vaskova M, Brdicka T, Fiser K, Hrusak O, Lund-Johansen F, Kalina T: High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells. Mol Cell Proteomics. 2016 Apr;15(4):1246-61., *Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98(5):1561-6. _x000D_ , *Drbal K, Angelisova P, Cerny J, Hilgert I, Horejsi V: A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins._x000D_ Immunobiology. 2001 May;203(4):687-98. _x000D_ , *Drbal K, Angelisova P, Cerny J, Pavlistova D, Cebecauer M, Novak P, Horejsi V: Human leukocytes contain a large pool of free forms of CD18. Biochem Biophys Res Commun. 2000 Aug 28;275(2):295-9. _x000D_ , *Aubert M, Yoon M, Sloan DD, Spear PG, Jerome KR: The virological synapse facilitates herpes simplex virus entry into T cells. J Virol. 2009 Jun;83(12):6171-83., *Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
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Properties
If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
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French translation
anticorps
