CD11a LFA-1 alpha chain monoclonal antibody

• Catalog number
  11-622-C100
• Price
  Please ask
• Size
  100ug

• Long name
  Rat Monoclonal Antibody against Mouse CD11a LFA-1 alpha chain, Clone: M17/4
• Antigen
  CD11a (mouse)
• Species reactivity
  Mouse (Mus musculus)
• Negative species reactivity
  at the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
• Clonality
  Monoclonal Antibody (Mab)
• Clone
  M17/4
• Applications
  FC, IP, IHC(F), FUNC
• Immunogen
  C57BL/6 mouse splenic secondary cytotoxic T lymphocytes_x000D_
• Format conjugation
  purified; unconjugated
• Specificity
  The rat monoclonal antibody M17/4 reacts with CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils._x000D_
• Research area
  CD and Related Antigens & MHC (Rodent)
• Concentration
  1 mg/ml
• Storage buffer
  Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Shipping and storage
  Rat Monoclonal Antibody against Mouse CD11a LFA-1 alpha chain, Clone: M17/4 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
• Background
  CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. _x000D_
• Purity
  > 95% (by SDS-PAGE)
• Purification method
  Purified by protein-A affinity chromatography
• Datasheet
• References
  _x000D_ , *Papayannopoulou T, Priestley GV, Nakamoto B, Zafiropoulos V, Scott LM, Harlan JM: Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2-deficient mice. Blood. 2001 Mar 1;97(5):1282-8._x000D_ , *Koseki S, Miura S, Fujimori H, Hokari R, Komoto S, Hara Y, Ogino T, Nagata H, Goto M, Hachimura S, Kaminogawa S, Ishii H: In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine. Int Immunol. 2001 Sep;13(9):1165-74._x000D_ , *Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J: The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo. Respir Res. 2005 Jul 12;6:70._x000D_ , *Bellingan GJ, Xu P, Cooksley H, Cauldwell H, Shock A, Bottoms S, Haslett C, Mutsaers SE, Laurent GJ: Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation. J Exp Med. 2002 Dec 2;196(11):1515-21._x000D_ , *Yung R, Powers D, Johnson K, Amento E, Carr D, Laing T, Yang J, Chang S, Hemati N, Richardson B: Mechanisms of drug-induced lupus. II. T cells overexpressing lymphocyte function-associated antigen 1 become autoreactive and cause a lupuslike disease in syngeneic mice. J Clin Invest. 1996 Jun 15;97(12):2866-71._x000D_ , *Smith SS, Barnum SR: Differential expression of beta 2-integrins and cytokine production between gammadelta and alphabeta T cells in experimental autoimmune encephalomyelitis. J Leukoc Biol. 2008 Jan;83(1):71-9._x000D_ , *Angelov GS, Guillaume P, Cebecauer M, Bosshard G, Dojcinovic D, Baumgaertner P, Luescher IF: Soluble MHC-peptide complexes containing long rigid linkers abolish CTL-mediated cytotoxicity. J Immunol. 2006 Mar 15;176(6):3356-65._x000D_ , *Kuhlman P, Moy VT, Lollo BA, Brian AA: The accessory function of murine intercellular adhesion molecule-1 in T lymphocyte activation. Contributions of adhesion and co-activation. J Immunol. 1991 Mar 15;146(6):1773-82._x000D_ , *Sanchez-Madrid F, Davignon D, Martz E, Springer TA: Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell Immunol. 1982 Oct;73(1):1-11._x000D_
• Description
  The Anti-CD11a LFA-1 alpha chain Mab is a α- or alpha protein sometimes glycoprotein present in blood.
• Properties
  If you buy Antibodies supplied by Exbio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  CD11a   LFA-1   alpha   chain   monoclonal  
• Gene symbol
  ITGAL, ITGB2, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1, MIR16-1
• Short name
  Anti-CD11a LFA-1 alpha chain Mab
• Technique
  Antibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
• Host
  Rat (Rattus norvegicus)
• Isotype
  IgG2a
• Alternative name
  Rat Monoclonal to CD11a (mouse) / LFA-1 alpha chain
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:6148
• Gene
  ITGAL
• Long gene name
  integrin subunit alpha L
• Synonyms gene
  • CD11A
• Synonyms gene name
  • integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
• Synonyms
  • LFA-1
  • LFA1A
• Synonyms name
  • antigen CD11A (p180)
  • lymphocyte function-associated antigen 1, alpha polypeptide
• Locus
  16p11.2
• Discovery year
  1986-01-01
• Entrez gene record
  3683
• Pubmed identfication
  • 3284962
• Classification
  • Integrin alpha subunits
  • CD molecules
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000176964

Gene info

• Identity
  HGNC:6155
• Gene
  ITGB2
• Long gene name
  integrin subunit beta 2
• Synonyms gene
  • CD18
  • MFI7
• Synonyms gene name
  • integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit)
  • integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
• Synonyms
  • LFA-1
  • MAC-1
• Synonyms name
  • complement component 3 receptor 3 and 4 subunit
• GenBank acession
  • AK222505
• Locus
  21q22.3
• Discovery year
  1986-01-01
• Entrez gene record
  3689
• RefSeq identity
  • NM_000211
• Classification
  • Integrin beta subunits
  • Complement system regulators and receptors
  • CD molecules
• VEGA ID
  OTTHUMG00000090257
• Locus Specific Databases
  • ITGB2base: Mutation registry for Leukocyte adhesion deficiency I (LAD-I)
  • LRG_76

Gene info

• Identity
  HGNC:5670
• Gene
  IGHVII-1-1
• Long gene name
  immunoglobulin heavy variable (II)-1-1 (pseudogene)
• Synonyms gene name
  • immunoglobulin heavy variable (II)-1-1
  • immunoglobulin heavy variable (II)-1-1 pseudogene
• Synonyms
  • IGHV(II)-1-1
• GenBank acession
  • AB019441
• Locus
  14q32.33
• Discovery year
  2000-04-17
• Entrez gene record
  28377
• RefSeq identity
  • NG_001019
• Classification
  • Immunoglobulin heavy locus at 14q32.33
• VEGA ID
  OTTHUMG00000152325

Gene info

• Identity
  HGNC:31499
• Gene
  MIR1-1
• Long gene name
  microRNA 1-1
• Synonyms gene
  • MIRN1-1
• Synonyms
  • hsa-mir-1-1
• Locus
  20q13.33
• Discovery year
  2004-04-23
• Entrez gene record
  406904
• RefSeq identity
  • NR_029780
• Classification
  • MicroRNA MIR1/206 family

Gene info

• Identity
  HGNC:50820
• Gene
  TRUND-NNN8-1
• Long gene name
  tRNA-undetermined (NNN) 8-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 8-1
• GenBank acession
  • HG984097
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482528
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:50819
• Gene
  TRUND-NNN7-1
• Long gene name
  tRNA-undetermined (NNN) 7-1
• Synonyms gene name
  • transfer RNA-undetermined (NNN) 7-1
• GenBank acession
  • HG984096
• Locus
  1
• Discovery year
  2014-06-20
• Entrez gene record
  103482531
• Pubmed identfication
  • 18984615

Gene info

• Identity
  HGNC:5760
• Gene
  IGKV1OR2-1
• Long gene name
  immunoglobulin kappa variable 1/OR2-1 (pseudogene)
• Synonyms gene
  • IGKVPZ1
  • IGKV1OR-1
• Synonyms gene name
  • immunoglobulin kappa variable 1/OR-1
  • immunoglobulin kappa variable 1/OR-1 pseudogene
  • immunoglobulin kappa variable 1/OR-1 (pseudogene)
• Synonyms
  • IGKV1/OR-1
  • IGKV1/OR2-1
• GenBank acession
  • Z12367
• Locus
  2p11.1
• Discovery year
  2000-04-18
• Entrez gene record
  3531
• Pubmed identfication
  • 2853098
• Classification
  • Immunoglobulin kappa (IGK) orphons
• VEGA ID
  OTTHUMG00000155081

Gene info

• Identity
  HGNC:31488
• Gene
  MIR101-1
• Long gene name
  microRNA 101-1
• Synonyms gene
  • MIRN101-1
• Synonyms
  • hsa-mir-101-1
• Locus
  1p31.3
• Discovery year
  2004-04-23
• Entrez gene record
  406893
• RefSeq identity
  • NR_029516
• Classification
  • MicroRNA MIR101 family

Gene info

• Identity
  HGNC:35281
• Gene
  MIR1289-1
• Long gene name
  microRNA 1289-1
• Synonyms gene
  • MIRN1289-1
• Synonyms
  • hsa-mir-1289-1
• Locus
  20q11.22
• Discovery year
  2008-11-03
• Entrez gene record
  100302125
• RefSeq identity
  • NR_031620
• Classification
  • MicroRNA MIR1289 family

Gene info

• Identity
  HGNC:31545
• Gene
  MIR16-1
• Long gene name
  microRNA 16-1
• Synonyms gene
  • MIRN16-1
• Synonyms
  • hsa-mir-16-1
• Locus
  13q14.2
• Discovery year
  2004-04-23
• Entrez gene record
  406950
• RefSeq identity
  • NR_029486
• Classification
  • MicroRNA MIR15/16 family

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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