- Catalog number1F-210-T025
- Product nameCD11a / LFA-1 alpha chain monoclonal antibody
- Size25 assays
- PriceAsk For Price
- Short nameAnti-CD11a / LFA-1 alpha chain Mab
- Long nameMouse Monoclonal Antibody against Human CD11a / LFA-1 alpha chain, Clone: MEM-25
- AntigenCD11a
- HostMouse (Mus musculus)
- Species reactivityHuman (Homo sapiens)
- Negative species reactivityat the time of upload there has been no data, please, contact us to obtain most recent information on species reactivity and cross reactivity
- ClonalityMonoclonal Antibody (Mab)
- CloneMEM-25
- IsotypeIgG1
- ApplicationsFC, IP, FUNC
- ImmunogenLeukocytes from a pacient suffering from a LGL-type leukaemia.
- Format conjugationFITC
- SpecificityThe antibody MEM-25 reacts with CD11a (alpha subunit of human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. _x000D_ HLDA IV; WS Code NL 209
- Research areaCD and Related Antigens (Human)
- Storage bufferThe reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide.
- Shipping and storageMouse Monoclonal Antibody against Human CD11a / LFA-1 alpha chain, Clone: MEM-25 is transported on blue ice/ice packs and should be stored at temperatures between 1 and 5 degrees Celsius. Do not freeze! Avoid exposing the product to direct light, especially the conjugated antibodies as most conjugates are very sentitive to light.
- BackgroundCD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
- Purity> 95% (by SDS-PAGE)
- Purification methodPurified by affinity chromatography
- Datasheet
- References, *Aubert M, Yoon M, Sloan DD, Spear PG, Jerome KR: The virological synapse facilitates herpes simplex virus entry into T cells. J Virol. 2009 Jun;83(12):6171-83., *Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym-1 antibody-dependent cytolysis by neutrophils exposed to granulocyte-macrophage colony-stimulating factor: intervention of FcgammaRII (CD32), CD11b-CD18 integrins, and CD66b glycoproteins. Blood. 1999 May 15;93(10):3505-11., *Mathison RD, Befus AD, Davison JS, Woodman RC: Modulation of neutrophil function by the tripeptide feG. BMC Immunol. 2003 Mar 4;4:3. Epub 2003 Mar 4., *Garnotel R, Rittié L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c-CD18, gp150-95). J Immunol. 2000 Jun 1;164(11):5928-34., *Wang JH, Kwas C, Wu L: Intercellular adhesion molecule 1 (ICAM-1), but not ICAM-2 and -3, is important for dendritic cell-mediated human immunodeficiency virus type 1 transmission. J Virol. 2009 May;83(9):4195-204., *Hajishengallis G, Martin M, Sojar HT, Sharma A, Schifferle RE, DeNardin E, Russell MW, Genco RJ: Dependence of bacterial protein adhesins on toll-like receptors for_x000D_ proinflammatory cytokine induction. Clin Diagn Lab Immunol. 2002 Mar;9(2):403-11., *Simon SI, Cherapanov V, Nadra I, Waddell TK, Seo SM, Wang Q, Doerschuk CM, Downey GP: Signaling functions of L-selectin in neutrophils: alterations in the cytoskeleton_x000D_ and colocalization with CD18. J Immunol. 1999 Sep 1;163(5):2891-901., *Ottonello L, Epstein AL, Mancini M, Dapino P, Dallegri F: Monoclonal LYM-1 antibody-dependent cytolysis by human neutrophils exposed to GM-CSF: auto-regulation of target cell attack by cathepsin G. J Leukoc Biol. 2004 Jan;75(1):99-105., *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989)., *Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V.: Monoclonal antibodies against human leucocyte antigens. IV. Antibodies against subunits of the LFA-1 (CD11a/CD18) leucocyte-adhesion glycoprotein. Folia Biol (Praha). 1990;36(1):41-50. _x000D_
- Gene targetCD11a / LFA-1 alpha chain monoclonal
- Identity:HGNC:6148
- Gene:ITGAL
- Long gene name:integrin subunit alpha L
- Synonyms gene name:integrin, alpha L (antigen CD11A (p180), lymphocyte function-associated antigen 1; alpha polypeptide)
- Synonyms:LFA-1LFA1A
- Discovery year:1986-01-01
- Entrez gene record:3683
- Pubmed identification:3284962
- Classification:Integrin alpha subunits, CD molecules, MicroRNA protein coding host genes
- VEGA ID:OTTHUMG00000176964
- Identity:HGNC:6155
- Gene:ITGB2
- Long gene name:integrin subunit beta 2
- Synonyms gene name:integrin, beta 2 (antigen CD18 (p95), lymphocyte function-associated antigen 1; macrophage antigen 1 (mac-1) beta subunit), integrin, beta 2 (complement component 3 receptor 3 and 4 subunit)
- Synonyms:LFA-1, MAC-1
- Discovery year:1986-01-01
- Entrez gene record:3689
- RefSeq identity:NM_000211
- Classification:Integrin beta subunits, Complement system regulators and receptors, CD molecules
- VEGA ID:OTTHUMG00000090257
- Identity:HGNC:5670
- Gene:IGHVII-1-1
- Long gene name:immunoglobulin heavy variable (II)-1-1 (pseudogene)
- Synonyms gene name:immunoglobulin heavy variable (II)-1-1, immunoglobulin heavy variable (II)-1-1 pseudogene
- Synonyms:IGHV(II)-1-1,
- Discovery year:2000-04-17
- Entrez gene record:28377
- RefSeq identity:NG_001019
- Classification:Immunoglobulin heavy locus at 14q32.33
- VEGA ID:OTTHUMG00000152325
- Identity:HGNC:31499
- Gene:MIR1-1
- Long gene name:microRNA 1-1
- Synonyms:hsa-mir-1-1
- Discovery year:2004-04-23
- Entrez gene record:406904
- RefSeq identity:NR_029780
- Classification:MicroRNA MIR1/206 family
- Identity:HGNC:50820
- Gene:TRUND-NNN8-1
- Long gene name:tRNA-undetermined (NNN) 8-1
- Synonyms gene name:transfer RNA-undetermined (NNN) 8-1
- Discovery year:2014-06-20
- Entrez gene record:103482528
- Pubmed identification:18984615
- Identity:HGNC:50819
- Gene:TRUND-NNN7-1
- Long gene name:tRNA-undetermined (NNN) 7-1
- Synonyms gene name:transfer RNA-undetermined (NNN) 7-1
- Discovery year:2014-06-20
- Entrez gene record:103482531
- Pubmed identification:18984615
- Identity:HGNC:5760
- Gene:IGKV1OR2-1
- Long gene name:immunoglobulin kappa variable 1/OR2-1 (pseudogene)
- Synonyms gene name:immunoglobulin kappa variable 1/OR-1, immunoglobulin kappa variable 1/OR-1 pseudogene, immunoglobulin kappa variable 1/OR-1 (pseudogene)
- Synonyms:IGKV1/OR-1, IGKV1/OR2-1,
- Discovery year:2000-04-18
- Entrez gene record:3531
- Pubmed identification:2853098
- Classification:Immunoglobulin kappa (IGK) orphons
- VEGA ID:OTTHUMG00000155081
- Identity:HGNC:31488
- Gene:MIR101-1
- Long gene name:microRNA 101-1
- Synonyms:hsa-mir-101-1
- Discovery year:2004-04-23
- Entrez gene record:406893
- RefSeq identity:NR_029516
- Classification:MicroRNA MIR101 family
- Identity:HGNC:35281
- Gene:MIR1289-1
- Long gene name:microRNA 1289-1
- Synonyms:hsa-mir-1289-1
- Discovery year:2008-11-03
- Entrez gene record:100302125
- RefSeq identity:NR_031620
- Classification:MicroRNA MIR1289 family
- Identity:HGNC:31545
- Gene:MIR16-1
- Long gene name:microRNA 16-1
- Synonyms:hsa-mir-16-1
- Discovery year:2004-04-23
- Entrez gene record:406950
- RefSeq identity:NR_029486
- Classification:MicroRNA MIR15/16 family
- Gene symbolITGAL, ITGB2, IGHVII-1-1, MIR1-1, TRUND-NNN8-1, TRUND-NNN7-1, IGKV1OR2-1, MIR101-1, MIR1289-1, MIR16-1
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- TechniqueAntibody, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies
- Alternative techniqueantibodies
Gene info
Gene info
Locus Specific Databases:ITGB2base: Mutation registry for Leukocyte adhesion deficiency I (LAD-I)LRG_76