phosphotyrosine FITC conjugate, Monoclonal Antibody

• Catalog number
  X1017S
• Price
  Please ask
• Size
  50µg

• Host Source
  Mouse
• Clone Name
  PY20
• Product Category
  Signal Transduction
• Species Reactivity
  Ubituitous
• Application
  Western Blot, Immunoprecipitation, Enzyme Immunoassay, Immunofluorescence
• Immunogen
  Hybridoma produced from Balb/C mice immunized with phosphotyrosine coupled to carrier protein.
• Purification Method
  NA
• Shipping Conditions
  Room Temperature
• Storage conditions
  phosphotyrosine FITC conjugate, Monoclonal Antibody should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles
• Datasheet link
• Properties
  If you buy Antibodies supplied by Exalpha they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• About
  Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
• French translation
  anticorps

• Gene target
  phosphotyrosine   conjugate   Monoclonal  
• Short name
  phosphotyrosine FITC conjugate, Monoclonal Antibody
• Technique
  Antibody, FITC, antibodies against human proteins, antibodies for, Fluorescein, Monoclonals or monoclonal antibodies
• Isotype
  IgG2b
• Label
  FITC
• Alternative name
  phosphotyrosine fluorecein conjugate, monoclonal (antibody to-)
• Alternative technique
  antibodies, fluorescine

MeSH Data

• Name
  Cytophotometry
• Concept
  Scope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
• Tree numbers
  • E01.370.225.500.386
  • E05.196.712.516.600.240
  • E05.200.500.386
  • E05.242.386
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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