Phosphotyrosine biotin conjugate

Products_type

Antibody
Products_short_name

[Phosphotyrosine]
Products_name_syn

[Mouse anti phosphotyrosine; PY20; Biotin Conjugate]
Clonality

Monoclonal
Clone

[PY20]
Reactivity

Ubituitous
Form

Provided as sterile filtered solution containing 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol at pH 7.5 and 3 mM sodium azide
Storage_stability

Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles
Tested_application

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA (EIA)
Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Conjugation

Biotinylated